Mouse anti-Human Prohibitin Monoclonal Antibody | anti-PHB antibody

Prohibitin (Mitochondrial Marker)

Cat. Num. AAA4381168

• Gene Names: PHB; PHB1; HEL-215; HEL-S-54e
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Prohibitin; Monoclonal Antibody; Prohibitin (Mitochondrial Marker); Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB; PHB1; anti-PHB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone Number: SPM311
• Specificity: Recognizes a protein of 30kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 272

• Applicable Applications for anti-PHB antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA); WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HepG2, A431 or MCF-7 cells. Liver, Kidney, Heart or Endometrium.
• Immunogen: Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic (Mitochondria)
• Hu-chromosome Location: 17q21.33
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Prohibitin Mouse Monoclonal Antibody (SPM311).)

SDS-Page

(SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (SPM311). Confirmation of Purity and Integrity of Antibody.)

References

McClung, J.K., Jupe, E.R., Liu, X-T., and Dell'Orco, R.T. Prohibitin: Potential role in senescence, development, and tumor suppression. Exp. Gerontol. 1995, 30(2):99-124.

NCBI and Uniprot Product Information

• NCBI GI #: 246483
• NCBI GeneID: 5245
• UniProt Accession #: P35232
• Molecular Weight: 30kDa
• NCBI Official Full Name: prohibitin
• NCBI Official Synonym Full Names: prohibitin
• NCBI Official Symbol: PHB
• NCBI Official Synonym Symbols: PHB1; HEL-215; HEL-S-54e
• NCBI Protein Information: prohibitin
• UniProt Protein Name: Prohibitin
• Protein Family: Prohibitin
• UniProt Gene Name: PHB
• UniProt Entry Name: PHB_HUMAN

NCBI Description

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.

Protein type: Transcription, coactivator/corepressor; Apoptosis; Oncoprotein; Mitochondrial; Chaperone; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; membrane; mitochondrion; integral to plasma membrane; mitochondrial inner membrane; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding

Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; regulation of apoptosis; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer