Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human heart stained with Prohibitin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of cardiac muscle cells.)
Mouse Prohibitin Monoclonal Antibody | anti-PHB antibody
Prohibitin antibody (biotin)
IP: 2 ug/ml protein lysate
WB: 0.5-1 ug/ml
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human heart stained with Prohibitin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of cardiac muscle cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human heart stained with Prohibitin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of cardiac muscle cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded rat heart stained with Prohibitin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of cardiac muscles)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded rat heart stained with Prohibitin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of cardiac muscles)
NCBI and Uniprot Product Information
NCBI Description
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.
Protein type: Chaperone; Mitochondrial; Motility/polarity/chemotaxis; Oncoprotein; Nuclear receptor co-regulator; Apoptosis; Cell cycle regulation; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: nucleoplasm; membrane; mitochondrion; integral to plasma membrane; cytoplasm; mitochondrial inner membrane; nucleus
Molecular Function: protein binding; enzyme binding; histone deacetylase binding
Biological Process: osteoblast differentiation; regulation of apoptosis; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; signal transduction
Disease: Breast Cancer
Research Articles on PHB
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Product Notes
The PHB phb (Catalog #AAA530665) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Prohibitin antibody (biotin) reacts with Chicken, Human, Mouse Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Prohibitin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB). IHC-F/p: 2-4 ug/ml IP: 2 ug/ml protein lysate WB: 0.5-1 ug/ml. Researchers should empirically determine the suitability of the PHB phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prohibitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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