Mouse anti-Human Progesterone Receptor Monoclonal Antibody | anti-PGR antibody

Progesterone Receptor, phosphorylated (Ser190)

Cat. Num. AAA631720

• Gene Names: PGR; PR; NR3C3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G chromatography
• Synonyms: Progesterone Receptor; Monoclonal Antibody; phosphorylated (Ser190); Anti -Progesterone Receptor; anti-PGR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6A166
• Specificity: Specific hPR phosphorylated at Ser190. On Western blots, it identifies a band at ~90kD representing the A-isoform and a band at ~115kD representing the B-isoform. Reactivity has been confirmed with human T47D cells.
• Purity/Purification: Affinity Purified; Purified by Protein G chromatography
• Form/Format: Supplied as a liquid in 10mM Hepes (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50%

• Applicable Applications for anti-PGR antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry and Western Blot.; Dilution: Immunohistochemistry: 1:1000; Western Blot: 1:1000
• Immunogen: Synthetic phospho peptide derived from a region of the human Progesterone Receptor (hPR) protein surrounding the phosphorylated Ser190 residue.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PGR antibody

Progesterone Receptor (PR) is phosphorylated on multiple serine residues located in the N-terminal domain and in the hinge region between the DNA and steroid binding domains. The activity of PR can be regulated by its phosphorylation by cyclin-A/Cdk2, casein kinase II, and MAP kinase. Individual sites such as Ser190 enhance transcriptional activity, while Ser294 seem to be a site of convergence and regulation by crosstalk with other signaling pathways. Of the seven identified serine residues on which the human PR (hPR) is phosphorylated, four (Ser81, Ser162, Ser190 and Ser400) are basally phosphorylated and exhibit a rapid increase in phosphorylation in response to treatment with hormone. The other three sites (Ser102, Ser294 and Ser345) are hormone-inducible and reach maximal phosphorylation within 1-2 hours of treatment.

Product Categories/Family for anti-PGR antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 189935
• NCBI GeneID: 5241
• UniProt Accession #: P06401; Q9UPF7; A7X8B0; Q6TZ07; Q6TZ08; Q8NG42; Q8NG43; Q8NG44; Q8NG45; Q8TDS3
• Molecular Weight: 98,981 Da
• NCBI Official Full Name: progesterone receptor
• NCBI Official Synonym Full Names: progesterone receptor
• NCBI Official Symbol: PGR
• NCBI Official Synonym Symbols: PR; NR3C3
• NCBI Protein Information: progesterone receptor; OTTHUMP00000236114; OTTHUMP00000236115; OTTHUMP00000236117; OTTHUMP00000236119; nuclear receptor subfamily 3 group C member 3
• UniProt Protein Name: Progesterone receptor
• Protein Family: Protein
• UniProt Gene Name: PGR
• UniProt Synonym Gene Names: NR3C3
• UniProt Entry Name: PRGR_HUMAN

NCBI Description

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq]

Uniprot Description

PR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; mitochondrial outer membrane

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; steroid hormone receptor activity; steroid binding; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; progesterone receptor signaling pathway; cell-cell signaling; epithelial cell maturation; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; ovulation from ovarian follicle; signal transduction; regulation of epithelial cell proliferation

Disease: Progesterone Resistance