Rabbit PRMT5 Monoclonal Antibody | anti-PRMT5 antibody

PRMT5 Rabbit Monoclonal Antibody

Cat. Num. AAA3014156

• Gene Names: PRMT5; HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: Protein A affinity purified
• Synonyms: PRMT5; Monoclonal Antibody; PRMT5 Rabbit Monoclonal Antibody; Protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs; HRMT1L5; IBP72; JBP1; SKB1; anti-PRMT5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of PRMT5 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 637

• Applicable Applications for anti-PRMT5 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:2000; ICC: 1:50-1:200; IF: 1:50-1:200; IHC: 1:50-1:200
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PRMT5 Rabbit mAb at 1:1000 dilutionLane1:A431 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining PRMT5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-PRMT5 antibody

PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 32171585
• NCBI GeneID: 10419
• UniProt Accession #: O14744
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: Protein arginine N-methyltransferase 5
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

Protein type: EC 2.1.1.125; Cell cycle regulation; Methyltransferase, protein arginine; RNA processing; Methyltransferase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity

Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; peptidyl-arginine N-methylation; endothelial cell activation