Western Blot (WB)
(Western Blot analysis of PRKG1 expression in transfected 293T cell line by PRKG1 monoclonal antibody. Lane 1: PRKG1 transfected lysate (35.5kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human PRKG1 Monoclonal Antibody | anti-PRKG1 antibody
PRKG1 (cGMP-dependent Protein Kinase 1, cGK 1, cGK1, cGMP-dependent Protein Kinase I, cGKI, PRKG1B, PRKGR1A, PRKGR1B, DKFZp686K042, FLJ36117, MGC71944) (FITC)
Gene Names
PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PRKG1; Monoclonal Antibody; PRKG1 (cGMP-dependent Protein Kinase 1; cGK 1; cGK1; cGMP-dependent Protein Kinase I; cGKI; PRKG1B; PRKGR1A; PRKGR1B; DKFZp686K042; FLJ36117; MGC71944) (FITC); anti-PRKG1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5E5
Specificity
Recognizes human PRKG1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-PRKG1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa73-170, from human PRKG1 (NP_006249) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
RTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGV
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of PRKG1 expression in transfected 293T cell line by PRKG1 monoclonal antibody. Lane 1: PRKG1 transfected lysate (35.5kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PRKG1 expression in transfected 293T cell line by PRKG1 monoclonal antibody. Lane 1: PRKG1 transfected lysate (35.5kD). Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged PRKG1 is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged PRKG1 is ~0.03ng/ml as a capture antibody.)
Related Product Information for anti-PRKG1 antibody
PKGs are cyclic GMP-dependent protein kinases (also designed cGKs) that are classified into two types, PKGI and PKGII. Studies have shown that cGKs and cyclic AMP-dependent kinases (cAKs) are highly homologous protein kinase families with similar substrate specificities. Phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility and platelet aggregation. However, recent studies using mice deficient in PKGs have shown that cGMP kinases regulate very specifically distinct pathways which are separate from those used by cyclic AMP kinases.
Product Categories/Family for anti-PRKG1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,185 Da
NCBI Official Full Name
cGMP-dependent protein kinase 1 isoform 2
NCBI Official Synonym Full Names
protein kinase, cGMP-dependent, type I
NCBI Official Symbol
PRKG1
NCBI Official Synonym Symbols
1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
NCBI Protein Information
cGMP-dependent protein kinase 1; protein kinase, cGMP-dependent, regulatory, type I, beta
UniProt Protein Name
cGMP-dependent protein kinase 1
Protein Family
UniProt Gene Name
PRKG1
UniProt Synonym Gene Names
PRKG1B; PRKGR1A; PRKGR1B; cGK 1; cGK1; cGKI
UniProt Entry Name
KGP1_HUMAN
Uniprot Description
PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.12; Protein kinase, AGC; AGC group; PKG family
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: Golgi apparatus; cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; calcium channel regulator activity; cGMP-dependent protein kinase activity; cGMP binding; ATP binding
Biological Process: regulation of GTPase activity; relaxation of vascular smooth muscle; cGMP-mediated signaling; forebrain development; dendrite development; neuron migration; signal transduction; blood coagulation; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation
Similar Products
Product Notes
The PRKG1 prkg1 (Catalog #AAA6149048) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRKG1 (cGMP-dependent Protein Kinase 1, cGK 1, cGK1, cGMP-dependent Protein Kinase I, cGKI, PRKG1B, PRKGR1A, PRKGR1B, DKFZp686K042, FLJ36117, MGC71944) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKG1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRKG1 prkg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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