Mouse PRKCE Monoclonal Antibody | anti-PRKCE antibody

PRKCE antibody

Cat. Num. AAA838034

• Gene Names: PRKCE; PKCE; nPKC-epsilon
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: PRKCE antibody was purified by affinity chromatography.
• Synonyms: PRKCE; Monoclonal Antibody; PRKCE antibody; Monoclonal PRKCE; Anti-PRKCE; Protein kinase C epsilon; PKCE; nPKC-epsilon; MGC125656; anti-PRKCE antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 4B3
• Purity/Purification: PRKCE antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 737

• Applicable Applications for anti-PRKCE antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100; IHC: 1:150; WB: 1:500-2000
• Biological Significance: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calciumand the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and areknown to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. this kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis.
• Cross-Reactivity: Human, Dog, Monkey
• Immunogen: PRKCE antibody was raised in mouse using a full length recombinant protein of human PRKCE (NP_005391) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PRKCE protein (Right) or empty vector (Left) detected with PRKCE antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PRKCE protein in paraffin embedded Human ovary tissue using PRKCE antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PRKCE protein using PRKCE antibody)

Related Product Information for anti-PRKCE antibody

Mouse monoclonal PRKCE antibody

Product Categories/Family for anti-PRKCE antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4885563
• NCBI GeneID: 5581
• NCBI Accession #: NP_005391.1
• NCBI GenBank Nucleotide #: NM_005400.2
• Molecular Weight: 83,674 Da
• NCBI Official Full Name: protein kinase C epsilon type
• NCBI Official Synonym Full Names: protein kinase C, epsilon
• NCBI Official Symbol: PRKCE
• NCBI Official Synonym Symbols: PKCE; nPKC-epsilon
• NCBI Protein Information: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCE
• UniProt Synonym Gene Names: PKCE
• UniProt Entry Name: KPCE_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Protein kinase, AGC; AGC group; PKC family; Eta subfamily

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: Golgi apparatus; cytoskeleton; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: actin monomer binding; calcium-independent protein kinase C activity; protein serine/threonine kinase activity; signal transducer activity; protein binding; protein kinase C activity; enzyme binding; metal ion binding; enzyme activator activity; ethanol binding; ATP binding; receptor activator activity

Biological Process: nerve growth factor receptor signaling pathway; apoptosis; response to morphine; positive regulation of lipid catabolic process; signal transduction; protein amino acid phosphorylation; positive regulation of MAPKKK cascade; lipopolysaccharide-mediated signaling pathway; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; positive regulation of cytokinesis; epidermal growth factor receptor signaling pathway; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; positive regulation of synaptic transmission, GABAergic; positive regulation of insulin secretion; cell cycle; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; phospholipase C activation; cell division; release of sequestered calcium ion into cytosol; macrophage activation during immune response; innate immune response; blood coagulation