Rabbit anti-Human CDK5R1 Polyclonal Antibody | anti-CDK5R1 antibody

CDK5R1(p35), CT (CDK5R1, CDK5R, NCK5A, Cyclin-dependent kinase 5 activator 1, Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunit, Cyclin-dependent kinase 5 activator 1, p35, Cyclin-dependent kinase 5 activator 1, p25, Tau protein kin

Cat. Num. AAA6284414

• Gene Names: CDK5R1; p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CDK5R1; Polyclonal Antibody; CDK5R1(p35); CT (CDK5R1; CDK5R; NCK5A; Cyclin-dependent kinase 5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; p35; p25; Tau protein kin; Tau protein kinase II 23kD subunit; anti-CDK5R1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CDK5R1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDK5R1(p35) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278~307 amino acids from the C-terminal region of human CDK5R1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5R1(p35) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(CDK5R1 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the CDK5R1 antibody detected the CDK5R1 protein (arrow).)

Related Product Information for anti-CDK5R1 antibody

p35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Product Categories/Family for anti-CDK5R1 antibody

Antibodies; Protein Kinases

References

Lin, S., FEBS Lett. 582 (8), 1197-1202 (2008). Sen, A., Neuroreport 18 (5), 511-516 (2007). Mitsios, N., Brain Pathol. 17 (1), 11-23 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4502737
• NCBI GeneID: 8851
• NCBI Accession #: NP_003876.1
• NCBI GenBank Nucleotide #: NM_003885.2
• UniProt Accession #: Q15078; Q5U0G3; E1P664
• Molecular Weight: 34,060 Da
• NCBI Official Full Name: cyclin-dependent kinase 5 activator 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5, regulatory subunit 1 (p35)
• NCBI Official Symbol: CDK5R1
• NCBI Official Synonym Symbols: p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
• NCBI Protein Information: cyclin-dependent kinase 5 activator 1; CDK5 activator 1; neuronal CDK5 activator; TPKII regulatory subunit; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; cyclin-dependent kinase 5 regulatory subunit 1
• UniProt Protein Name: Cyclin-dependent kinase 5 activator 1
• Protein Family: Cyclin-dependent kinase 5 activator
• UniProt Gene Name: CDK5R1
• UniProt Synonym Gene Names: CDK5R; NCK5A; CDK5 activator 1; p35; p25; p23
• UniProt Entry Name: CD5R1_HUMAN

NCBI Description

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK5R1: activator of cyclin-dependent kinase 5 (CDK5). Required for proper development of the central nervous system. The p35 form of this protein is cleaved by calpain, generating a p25 form, resulting in the prolonged activation and relocalization of the CDK5 from the cell periphery to nuclear and perinuclear regions. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease, leading to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: intracellular membrane-bound organelle; contractile fiber; cyclin-dependent protein kinase 5 activator complex; dendrite; postsynaptic density; dendritic spine; cytosol; nucleoplasm; growth cone; cell soma; membrane; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; neuromuscular junction; nucleus

Molecular Function: protein serine/threonine kinase activator activity; protein binding; ephrin receptor binding; cadherin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase 5 activator activity; calcium ion binding; kinase activity; protein kinase binding; protein kinase activity

Biological Process: axon guidance; superior olivary nucleus maturation; acetylcholine receptor signaling, muscarinic pathway; regulation of neuron differentiation; hippocampus development; rhythmic process; axonal fasciculation; neuron migration; peptidyl-threonine phosphorylation; negative regulation of axon extension; neuron differentiation; cell proliferation; embryonic development; peptidyl-serine phosphorylation; ionotropic glutamate receptor signaling pathway; positive regulation of neuron apoptosis; regulation of actin cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; cerebellum development; neuron adhesion; regulation of cyclin-dependent protein kinase activity; brain development; negative regulation of transcription, DNA-dependent; neurite development; layer formation in the cerebral cortex; serine phosphorylation of STAT3 protein