Mouse anti-Human Pregnancy Associated Plasma Protein A Monoclonal Antibody | anti-PAPP-A antibody
Pregnancy Associated Plasma Protein A (PAPP-A, Pappalysin 1, PAPA, PAPPA, PAPP-A, PAPPA1, Aspecific BCL2 ARE-binding Protein 2, ASBABP2, Differentially Placenta 1 Expressed Protein, DIPLA1, EC 3.4.24.79, IGF-dependent IGFBP-4 Protease, IGFBP-4ase, Insulin
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Pregnancy Associated Plasma Protein A; Monoclonal Antibody; Pregnancy Associated Plasma Protein A (PAPP-A; Pappalysin 1; PAPA; PAPPA; PAPP-A; PAPPA1; Aspecific BCL2 ARE-binding Protein 2; ASBABP2; Differentially Placenta 1 Expressed Protein; DIPLA1; EC 3.4.24.79; IGF-dependent IGFBP-4 Protease; IGFBP-4ase; Insulin; anti-PAPP-A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
5E743
Specificity
Specific to the proMBP subunit of heterotetrameric PAPP-A (htPAPP-A; PAPP-A/proMBP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PAPP-A antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Detection of human PAPP-A antigen in ELISA, Western Blot and Western Blot. Recognizes PAPP-A subunit in the heterotetrameric complex in Western Blot. Recognizes proMBP subunit in Western Blot.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Human PAPP-A antigen purified from placental blood is a heterotetrameric complex, consisting of two PAPP-A subunits and two pro-MBP subunits). Human PAPP-A antigen purified from atherosclerotic plaques is homodimeric consisting of 2 PAPP-A subunits.
Conjugate
PE
Hybridoma
Derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP complex.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Related Product Information for anti-PAPP-A antibody
PAPP-A was initially identified as a high molecular mass component in human pregnancy serum. In pregnancy serum PAPP-A exists in a disulfide bound heterotetrameric complex consisting of two 200kD PAPP-A subunits and two 50-90kD subunits of the proform of eosinophil major basic protein (proMBP). The concentration of PAPP-A in serum increases with gestational age, however, in Down's syndrome pregnancies PAPP-A concentration is markedly reduced in the first trimester. As such, PAPP-A is widely used as an early marker for Down's syndrome. PAPP-A has also been shown to exist in a homodimeric, 400kD form in unstable atherosclerotic plaques. Increased circulating concentrations of this dimeric PAPP-A are associated with acute coronary syndromes.
Product Categories/Family for anti-PAPP-A antibody
Similar Products
Product Notes
The PAPP-A (Catalog #AAA6129535) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Pregnancy Associated Plasma Protein A (PAPP-A, Pappalysin 1, PAPA, PAPPA, PAPP-A, PAPPA1, Aspecific BCL2 ARE-binding Protein 2, ASBABP2, Differentially Placenta 1 Expressed Protein, DIPLA1, EC 3.4.24.79, IGF-dependent IGFBP-4 Protease, IGFBP-4ase, Insulin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pregnancy Associated Plasma Protein A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Detection of human PAPP-An antigen in ELISA, Western Blot and Western Blot. Recognizes PAPP-A subunit in the heterotetrameric complex in Western Blot. Recognizes proMBP subunit in Western Blot. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PAPP-A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pregnancy Associated Plasma Protein A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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