Mouse anti-Human PP1A/PPP1CA Monoclonal Antibody | anti-PP1A antibody

PP1A/PPP1CA (N-terminus) Monoclonal Antibody

Cat. Num. AAA3014270

• Gene Names: PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PP1A/PPP1CA; Monoclonal Antibody; PP1A/PPP1CA (N-terminus) Monoclonal Antibody; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A; PPP1CA; PPP1A; anti-PP1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: This antibody detects endogenous levels of PP1A (N-terminus) and does not cross-react with related proteins
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 341

• Applicable Applications for anti-PP1A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:200-400; IHC: 1:500
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human PP1A / PPP1CA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot detection of PPP1CA in MCF7, K562, Hela, HEPG2 &Jurkat cell lysates using PPP1CA antibody (1:1000 diluted).)

Immunohistochemistry (IHC)

(IHC of paraffin-embedded human breast cancer using anti- PPP1CA diluted 1/500-1/1000.)

Immunocytochemistry (ICC)

(Immunocytochemistry stain of Hela using PPP1CA antibody (1:300).)

Related Product Information for anti-PP1A antibody

Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role. in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.

NCBI and Uniprot Product Information

• NCBI GI #: 56790945
• NCBI GeneID: 5499
• NCBI Accession #: NP_001008709.1
• NCBI GenBank Nucleotide #: NP_001008709.1
• UniProt Accession #: P62136
• Molecular Weight: Predicted Band Size: 38KDa ; Observed Band Size: 38KDa
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 3
• NCBI Official Synonym Full Names: protein phosphatase 1 catalytic subunit alpha
• NCBI Official Symbol: PPP1CA
• NCBI Official Synonym Symbols: PP1A; PP-1A; PPP1A; PP1alpha
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Gene Name: PPP1CA
• UniProt Synonym Gene Names: PPP1A; PP-1A
• UniProt Entry Name: PP1A_HUMAN

NCBI Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP1CA: is a catalytic subunit of protein phosphatase 1 (PP1), aubiquitous cytosolic serine-threonine phosphatase. Composed of a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Nucleolus

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; glycogen granule; cytoplasm; nucleolus; plasma membrane; dendritic spine; perikaryon; protein phosphatase type 1 complex; nucleus; cytosol

Molecular Function: ribonucleoprotein binding; protein binding; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity

Biological Process: glycogen metabolic process; regulation of glycogen catabolic process; branching morphogenesis of a tube; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; regulation of glycogen biosynthetic process; circadian regulation of gene expression; cell cycle; regulation of circadian rhythm; protein amino acid dephosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; entrainment of circadian clock by photoperiod; lung development