Human Prothrombin Fragment 1+2 (F1+2) ELISA Kit | F1+2 elisa kit

Human Prothrombin Fragment 1+2 (F1+2) High Sensitive ELISA Kit

Cat. Num. AAA2087036

• Gene Names: F2; PT; THPH1; RPRGL2
• Reactivity: Human
• Synonyms: Prothrombin Fragment 1+2 (F1+2); Human Prothrombin Fragment 1+2 (F1+2) High Sensitive ELISA Kit; F1+2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Prothrombin Fragment 1+2 (F1+2).; No significant cross-reactivity or interference between High Sensitive Prothrombin Fragment 1+2 (F1+2) and analogues was observed.
• Sequence Length: 622

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.11-80pg/mL
• Sensitivity: < 0.04pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F1+2 elisa kit

The microplate provided in this kit has been pre-coated with an antibody specific to F1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F1+2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503635
• NCBI GeneID: 2147
• NCBI Accession #: NP_000497.1
• NCBI GenBank Nucleotide #: NM_000506.4
• UniProt Accession #: P00734; Q4QZ40; Q53H04; Q53H06; Q69EZ7; Q7Z7P3; Q9UCA1; B2R7F7; B4E1A7
• Molecular Weight: 70,037 Da
• NCBI Official Full Name: prothrombin isoform 1 preproprotein
• NCBI Official Synonym Full Names: coagulation factor II, thrombin
• NCBI Official Symbol: F2
• NCBI Official Synonym Symbols: PT; THPH1; RPRGL2
• NCBI Protein Information: prothrombin
• UniProt Protein Name: Prothrombin
• UniProt Gene Name: F2

NCBI Description

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.