Mouse Poly Monoclonal Antibody | anti-Poly antibody
Poly (ADP-ribose) monoclonal antibody (10H)
Reactivity
Human, Mouse, Rat, Drosophila
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A-Affinity purified from supernatant
Synonyms
Poly; Monoclonal Antibody; Poly (ADP-ribose) monoclonal antibody (10H); anti-Poly antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Drosophila
Clonality
Monoclonal
Isotype
IgG3
Clone Number
10H
Specificity
Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme
Purity/Purification
Protein A-Affinity purified from supernatant
Form/Format
Liquid. In 50mM HEPES, pH7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide
Applicable Applications for anti-Poly antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Immunogen
Purified poly(ADP-ribose)
Preparation and Storage
For long term storage, store at -20 degree C. Avoid freeze/thaw cycles
Shipping: Shipped on Blue Ice
Shipping: Shipped on Blue Ice
Related Product Information for anti-Poly antibody
The monoclonal antibody 10H is directed against poly(ADP-ribose) (PAR). PAR is synthesized after activation of the nuclear DNA repair enzyme poly(ADP-ribose)polymerase (PARP). PARP is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself.The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP-ribose)glycohydrolase (PARG). After massive DNA damage (e.g. gamma-irradiation or oxidative stress) PAR is detectable in the first 10 minutes and disappears later on. In keratinocytes MAb 10H has been shown to detect UVB-induced apoptosis as early as 4 hour after irradiation, thus being superior to DNA laddering and the TUNEL assay.Due to the very large number of endonuclease-mediated DNA breaks in apoptosis, PARP becomes strongly activated during the so-called execution phase. In the case of DNA damage-induced apoptosis, this represents a "second round" of PAR synthesis. PAR synthesized during apoptosis appears to be remarkably stable. PAR immunofluorescence appears at least as early during apoptosis as does the specific cleavage of PARP by caspase-3. As shown by several groups, this PAR immunofluorescence correlates well with other markers of apoptosis. MAb to Poly(ADP-ribose) (10H) can be used in flow cytometry.A quantitative non-isotopic immuno-dot-blot method for the assessment of cellular poly(ADP-ribosyl)ation capacity using MAb to Poly(ADP-ribose) (10H) has been described.
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Product Notes
The Poly (Catalog #AAA566319) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Poly (ADP-ribose) monoclonal antibody (10H) reacts with Human, Mouse, Rat, Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's Poly can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). Researchers should empirically determine the suitability of the Poly for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Poly, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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