Testing Data
(Staining of paraffin embedded human tonsil with Mouse anti Human CD72 (MBS212848))
Mouse CD72 Monoclonal Antibody | anti-CD72 antibody
MOUSE ANTI HUMAN CD72
Gene Names
CD72; LYB2; CD72b
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
Synonyms
CD72; Monoclonal Antibody; MOUSE ANTI HUMAN CD72; anti-CD72 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
Bu40
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
359
Applicable Applications for anti-CD72 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Immunohistology - Paraffin: Maximum Dilution: 1/50; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Successful staining has also been accomplished using heat mediated antigen retrieval in citrate buffer pH6.0, see Lorenzi et al. for details
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Immunohistology - Paraffin: Maximum Dilution: 1/50; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Successful staining has also been accomplished using heat mediated antigen retrieval in citrate buffer pH6.0, see Lorenzi et al. for details
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of paraffin embedded human tonsil with Mouse anti Human CD72 (MBS212848))
Testing Data
(Staining of paraffin embedded human tonsil with Mouse anti Human CD72 (MBS212848))
Related Product Information for anti-CD72 antibody
Mouse anti Human CD72 antibody, clone Bu40 recognizes human CD72, a 86kD cell surface homodimer which is expressed on B lymphocytes. CD72 is expressed on early B cell precursors, through to mature activated B cells, but is lost upon terminal differentiation to plasma cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,220 Da
NCBI Official Full Name
B-cell differentiation antigen CD72
NCBI Official Synonym Full Names
CD72 molecule
NCBI Official Symbol
CD72
NCBI Official Synonym Symbols
LYB2; CD72b
NCBI Protein Information
B-cell differentiation antigen CD72; CD72 antigen; lyb-2
UniProt Protein Name
B-cell differentiation antigen CD72
Protein Family
UniProt Gene Name
CD72
UniProt Entry Name
CD72_HUMAN
Uniprot Description
CD72: Plays a role in B-cell proliferation and differentiation.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding; receptor binding
Biological Process: axon guidance; signal transduction; cell adhesion
Research Articles on CD72
Similar Products
Product Notes
The CD72 cd72 (Catalog #AAA216178) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD72 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100 Immunohistology - Paraffin: Maximum Dilution: 1/50; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Successful staining has also been accomplished using heat mediated antigen retrieval in citrate buffer pH6.0, see Lorenzi et al. for details. Researchers should empirically determine the suitability of the CD72 cd72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD72, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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