Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PLK1 protein (red) or empty vector (blue) stained using PLK1 antibody)
Mouse PLK1 Monoclonal Antibody | anti-PLK1 antibody
PLK1 antibody
Applications
Western Blot
Purity
PLK1 antibody was purified by affinity chromatography.
Synonyms
PLK1; Monoclonal Antibody; PLK1 antibody; Monoclonal PLK1; Anti-PLK1; Polo-Like Kinase 1; PLK; STPK13; PLK 1; PLK-1; anti-PLK1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C12
Purity/Purification
PLK1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
603
Applicable Applications for anti-PLK1 antibody
Western Blot (WB)
Application Notes
WB: 1:2000
Biological Significance
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the in activation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.
Cross-Reactivity
Human, Monkey, Dog
Immunogen
PLK1 antibody was raised in mouse using a protein expressed in 293T cell transfected with human PLK1 expression vector as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PLK1 protein (red) or empty vector (blue) stained using PLK1 antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PLK1 protein (red) or empty vector (blue) stained using PLK1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PLK1 protein (Right) or empty vector (Left) detected with PLK1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PLK1 protein (Right) or empty vector (Left) detected with PLK1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PLK1 protein using PLK1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PLK1 protein using PLK1 antibody)
Related Product Information for anti-PLK1 antibody
Mouse monoclonal PLK1 antibody
Product Categories/Family for anti-PLK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
PLK1, partial
Protein Family
Similar Products
Product Notes
The PLK1 (Catalog #AAA837933) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PLK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2000. Researchers should empirically determine the suitability of the PLK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
