Rabbit anti-Human GPR50 Polyclonal Antibody | anti-GPR50 antibody

GPR50, ID (GPR50, Melatonin-related receptor, G protein-coupled receptor 50, H9) (APC)

Cat. Num. AAA6303943

• Gene Names: GPR50; H9; Mel1c
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPR50; Polyclonal Antibody; ID (GPR50; Melatonin-related receptor; G protein-coupled receptor 50; H9) (APC); H9; anti-GPR50 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-GPR50 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPR50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323~352 amino acids from the Center region of human GPR50.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GPR50 Antibody (Center) in K562 cell line lysates (35ug/lane). GPR50 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(GPR50 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GPR50 antibody

Melatonin-related receptor expression has been documented from human CNS tissues, specifically in hypothalamus and pituitary, but not in human peripheral tissues. In animals, expression has also been seen in the CNS, and peripheral tissues.

Product Categories/Family for anti-GPR50 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 150170722
• NCBI GeneID: 9248
• NCBI Accession #: NP_004215.2
• NCBI GenBank Nucleotide #: NM_004224.3
• UniProt Accession #: Q13585; Q0VGG3; Q3ZAR0
• Molecular Weight: 67,369 Da
• NCBI Official Full Name: melatonin-related receptor
• NCBI Official Synonym Full Names: G protein-coupled receptor 50
• NCBI Official Symbol: GPR50
• NCBI Official Synonym Symbols: H9; Mel1c
• NCBI Protein Information: melatonin-related receptor
• UniProt Protein Name: Melatonin-related receptor
• Protein Family: Melatonin-related receptor
• UniProt Gene Name: GPR50
• UniProt Entry Name: MTR1L_HUMAN

NCBI Description

This gene product belongs to the G-protein coupled receptor 1 family. Even though this protein shares similarity with the melatonin receptors, it does not bind melatonin, however, it inhibits melatonin receptor 1A function through heterodimerization. Polymorphic variants of this gene have been associated with bipolar affective disorder in women. [provided by RefSeq, Jan 2010]

Uniprot Description

GPR50: an orphan G protein coupled receptor with high sequence homology to the melatonin receptors MT1 and MT2. Does not bind melatonin. Forms heterodimers with MT1 and MT2 and acts as a negative regulator of MT1 agonist binding and G protein coupling; inhibition of melatonin receptor activity is dependent on the long, proline-rich carboxy terminal tail of GPR50. On a physiological level, GPR50 is involved in the regulation of adaptive thermoregulation in mammals and deletion of GPR50 in mice produces a profound effect on the response to fasting and facilitates entry into torpor. Polymorphisms in the corresponding GPR50 gene are associated with bipolar affective disorder and major depressive disorder in women, indicating that variation in GPR50 may be an important gender-specific risk factor for certain mental disorders. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; identical protein binding; protein binding; melatonin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling