Mouse anti-Human PIP4K2A / PIPK Monoclonal Antibody | anti-PIP4K2A antibody

Mouse Monoclonal [clone 3A3] (IgG1,k) to Human PIP4K2A / PIPK

Cat. Num. AAA246345

• Gene Names: PIP4K2A; PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: PIP4K2A / PIPK; Monoclonal Antibody; Mouse Monoclonal [clone 3A3] (IgG1; k) to Human PIP4K2A / PIPK; Anti-PIP4K2A / PIPK Antibody (clone 3A3) IHC-plus; PIP4K2A; PI(5)P 4-kinase type II alpha; PIP5KII-alpha; PIP5KIII; PIPK; PtdIns(4)P-5-kinase B isoform; PtdIns(4)P-5-kinase C isoform; PI5P4KA; PIP4KII-alpha; PIP5K2; PIP5KIIalpha; PIP5K2A; PIP5KIIA; Human PIP4K2A; anti-PIP4K2A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3A3
• Specificity: Human PIP5K2A
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.48 mg/ml (varies by lot)
• Sequence Length: 406

• Applicable Applications for anti-PIP4K2A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: PIP5K2A (NP_005019, 304 a.a. ~ 366 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PIP4K2A / PIPK antibody was raised against pIP5K2A (NP_005019, 304 a.a. ~ 366 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PIP4K2A / PIP5K2A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(PIP5K2A monoclonal antibody Western blot of PIP5K2A expression in K-562.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PIP5K2A is approximately 3 ng/ml as a capture antibody.)

Product Categories/Family for anti-PIP4K2A antibody

Family: Non-Protein Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 6857820
• NCBI GeneID: 5305
• NCBI Accession #: NP_005019.2
• NCBI GenBank Nucleotide #: NM_005028.4
• UniProt Accession #: P48426; P53807; Q5VUX3; B0YJ66; B4DGX2; D3DRV1
• Molecular Weight: 39,802 Da
• NCBI Official Full Name: phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
• NCBI Official Synonym Full Names: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• NCBI Official Symbol: PIP4K2A
• NCBI Official Synonym Symbols: PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
• NCBI Protein Information: phosphatidylinositol 5-phosphate 4-kinase type-2 alpha; PIP5KIII; PIP5KIIalpha; PIP4KII-alpha; PI(5)P 4-kinase type II alpha; PtdIns(4)P-5-kinase B isoform; ptdIns(4)P-5-kinase C isoform; diphosphoinositide kinase 2-alpha; ptdIns(5)P-4-kinase isoform 2-alpha; 1-phosphatidylinositol-4-phosphate kinase; 1-phosphatidylinositol-4-phosphate-5-kinase; 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha; phosphatidylinositol-5-phosphate 4-kinase type-2 alpha; phosphatidylinositol 5-phosphate 4-kinase type II alpha; phosphatidylinositol-4-phosphate 5-kinase, type II, alpha; type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform
• UniProt Protein Name: Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
• Protein Family: Phosphatidylinositol 5-phosphate 4-kinase
• UniProt Gene Name: PIP4K2A
• UniProt Synonym Gene Names: PIP5K2; PIP5K2A; PI(5)P 4-kinase type II alpha; PIP4KII-alpha
• UniProt Entry Name: PI42A_HUMAN

NCBI Description

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]

Uniprot Description

PIP4K2A: Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

Protein type: EC 2.7.1.149; Motility/polarity/chemotaxis; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: plasma membrane; cytosol; nucleus

Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; ATP binding

Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process