Mouse anti-Human PIp1 Monoclonal Antibody | anti-PIP1 antibody

PIp1, ID (Proteolipid protein1)

Cat. Num. AAA6001945

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: PIp1; Monoclonal Antibody; ID (Proteolipid protein1); Anti -PIp1; anti-PIP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 538CT16.5.4
• Specificity: Human
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-PIP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA.; Dilution: ELISA: 1:1,000; Western Blot: 1:100~500
• Immunogen: RAT PIp1 Mab is generated from mouses immunized with a KLH conjugated synthetic peptide selected from human RAT PIp1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) using MBS6001945. This demonstrates theat MBS6001945 detected the PIp1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) using MBS6001945. This demonstrates that MBS6001945 detected the PIp1 protein (arrow).)

Testing Data

(All lanes: MBS6001945 (1:1000) Lane 1: C6 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: mouse cerebellum lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-mouse IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 30kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PIP1 antibody

This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. Proteolipid protein (Plp) gene mutation in rodents causes severe CNS dysmyelination, early death, and lethal hypoxic ventilatory depression (Miller et al. 2004). Myelin proteolipid protein (PLP) is the major protein in CNS myelin, accounting for 50% of myelin-associated proteins in the central nervous system. Mutations in the Plp gene, which are X-linked, have been found in rodents, such as the myelin deficient (MD) rat, and the myelin synthesis deficient mouse (Plpmsd). These mutations cause severe dysmyelination in the CNS, associated with oligodendrocyte death and non-myelinated or poorly myelinated axons, as well as abnormal axoglial junctions (Dentinger et al. 1982; Rosenbluth 1987). Most rodents with mutations in the Plp gene develop tremors, ataxia, and seizures in the second week of life, and early death by postnatal day (p) 21-23. (Knapp 1996; Garbern et al. 1999; Yool et al. 2000). Plp mutations in humans are associated with a leukoencephalopathy, Pelizaeus-Merzbacher disease, which bears many pathophysiologic similarities to the rodents with Plp mutations (Boulloche and Aicardi 1986). The mechanisms by which mutations in the Plp gene cause a complex and potentially fatal leukoencephalopathy are incompletely understood.

Product Categories/Family for anti-PIP1 antibody

Antibodies; Abs to Myelin

NCBI and Uniprot Product Information

• NCBI GI #: 351722655
• NCBI GeneID: 547857
• NCBI Accession #: NP_001235204.1
• NCBI GenBank Nucleotide #: NM_001248275.1
• NCBI Official Full Name: Pip1 protein
• NCBI Official Symbol: PIP1
• NCBI Protein Information: Pip1 protein
• Protein Family: RING-box protein