Mouse anti-Human GJC2 Monoclonal Antibody | anti-GJC2 antibody

GJC2, NT (GJC2, GJA12, Gap junction gamma-2 protein, Connexin-46.6, Connexin-47, Gap junction alpha-12 protein)

Cat. Num. AAA648279

• Gene Names: GJC2; Cx47; HLD2; GJA12; SPG44; CX46.6; LMPH1C; PMLDAR
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: GJC2; Monoclonal Antibody; NT (GJC2; GJA12; Gap junction gamma-2 protein; Connexin-46.6; Connexin-47; Gap junction alpha-12 protein); Anti -GJC2; anti-GJC2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 391CT6.4.3
• Specificity: Human
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-GJC2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA.; Dilution: ELISA: 1:1,000; Western Blot: 1:100~500
• Immunogen: GJC2 Mab is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the N-term region of human GJC2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(GJC2 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GJC2 antibody detected the GJC2 protein (arrow).)

Related Product Information for anti-GJC2 antibody

GJC2 is a gap junction protein. Gap junction proteins are members of a large family of homologous connexins and comprise 4 transmembrane, 2 extracellular, and 3 cytoplasmic domains. This gene plays a key role in central myelination and is involved in peripheral myelination in humans. Defects in this gene are the cause of autosomal recessive Pelizaeus-Merzbacher-like disease-1.

Product Categories/Family for anti-GJC2 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 45439367
• NCBI GeneID: 57165
• NCBI Accession #: NP_065168.2
• NCBI GenBank Nucleotide #: NM_020435.3
• UniProt Accession #: Q5T442; O43440; Q7Z7J2; Q8IWJ9
• Molecular Weight: 47,002 Da
• NCBI Official Full Name: gap junction gamma-2 protein
• NCBI Official Synonym Full Names: gap junction protein, gamma 2, 47kDa
• NCBI Official Symbol: GJC2
• NCBI Official Synonym Symbols: Cx47; HLD2; GJA12; SPG44; CX46.6; LMPH1C; PMLDAR
• NCBI Protein Information: gap junction gamma-2 protein; connexin-47; connexin-46.6; gap junction alpha-12 protein
• UniProt Protein Name: Gap junction gamma-2 protein
• Protein Family: Gap junction gamma-2 protein
• UniProt Gene Name: GJC2
• UniProt Synonym Gene Names: GJA12; Cx46.6; Cx47
• UniProt Entry Name: CXG2_HUMAN

NCBI Description

This gene encodes a gap junction protein. Gap junction proteins are members of a large family of homologous connexins and comprise 4 transmembrane, 2 extracellular, and 3 cytoplasmic domains. This gene plays a key role in central myelination and is involved in peripheral myelination in humans. Defects in this gene are the cause of autosomal recessive Pelizaeus-Merzbacher-like disease-1. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a role in myelination in central and peripheral nervous systems. Ref.5

Subunit structure: A connexon is composed of a hexamer of connexins. Interacts with TJP1

By similarity.

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.

Tissue specificity: Expressed in central nervous system, in sciatic nerve and sural nerve. Also detected in skeletal muscles. Ref.5

Involvement in disease: Leukodystrophy, hypomyelinating, 2 (HLD2) [MIM:608804]: An autosomal recessive hypomyelinating leukodystrophy with symptoms of Pelizaeus-Merzbacher disease. Clinically characterized by nystagmus, impaired motor development, ataxia, choreoathetotic movements, dysarthria, and progressive spasticity.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5Spastic paraplegia 44, autosomal recessive (SPG44) [MIM:613206]: A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG39 is associated with a motor axonopathy affecting upper and lower limbs and resulting in progressive wasting of distal upper and lower extremity muscles.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5 Ref.7Lymphedema, hereditary, 1C (LMPH1C) [MIM:613480]: A chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5 Ref.8

Sequence similarities: Belongs to the connexin family. Gamma-type subfamily.

Caution: It is uncertain whether Met-1 or Met-4 is the initiator.

Sequence caution: The sequence AAB94511.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH35840.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.