Mouse PINK1 Monoclonal Antibody | anti-PINK1 antibody

PINK1 Antibody: FITC

Cat. Num. AAA803746

• Gene Names: PINK1; BRPK; PARK6
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Synonyms: PINK1; Monoclonal Antibody; PINK1 Antibody: FITC; Parkinson disease (autosomal recessive) 6; EC 2.7.11.1; PARK6; serine/threonine-protein kinase PINK1 mitochondrial; PTEN-induced putative kinase protein 1; protein kinase BRPK; BRPK; FLJ27236; PTEN induced putative kinase 1; Phosphatase and Tensin Homolog; anti-PINK1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S4-15
• Form/Format: Protein G Purified
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 581

• Applicable Applications for anti-PINK1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: 1:1000 WB
• Conjugate: FITC
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western Blot analysis of Rat Brain showing detection of ~50 kDa PINK1 protein using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.)

Related Product Information for anti-PINK1 antibody

Background Info: Detects a 50 kDa protein corresponding to the molecular weight of PINK1

Scientific Background: PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.

Product Categories/Family for anti-PINK1 antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 14165272
• NCBI GeneID: 65018
• NCBI Accession #: NP_115785.1
• NCBI GenBank Nucleotide #: NM_032409.2
• UniProt Accession #: Q9BXM7; Q8N6T9; Q8NBU3; Q96DE4
• Molecular Weight: 30,104 Da
• NCBI Official Full Name: serine/threonine-protein kinase PINK1, mitochondrial
• NCBI Official Synonym Full Names: PTEN induced putative kinase 1
• NCBI Official Symbol: PINK1
• NCBI Official Synonym Symbols: BRPK; PARK6
• NCBI Protein Information: serine/threonine-protein kinase PINK1, mitochondrial; protein kinase BRPK; PTEN-induced putative kinase protein 1
• UniProt Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PINK1
• UniProt Entry Name: PINK1_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

Uniprot Description

PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, Other; Mitochondrial; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NKF2 family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrion; mitochondrial intermembrane space; cytosol; mitochondrial outer membrane; cytoskeleton; membrane; axon; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; TORC2 complex; nucleus; chromatin; integral to mitochondrial outer membrane

Molecular Function: protein kinase B binding; protein serine/threonine kinase activity; protein binding; protease activator activity; protease binding; ubiquitin protein ligase binding; magnesium ion binding; kinase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of JNK cascade; positive regulation of dopamine secretion; positive regulation of translation; regulation of protein ubiquitination; protein ubiquitination; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of macroautophagy; regulation of mitochondrial membrane potential; positive regulation of ubiquitin-protein ligase activity; response to stress; regulation of protein complex assembly; negative regulation of neuron apoptosis; regulation of hydrogen peroxide metabolic process; mitochondrion organization and biogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; activation of protein kinase B; positive regulation of synaptic transmission, dopaminergic; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; mitochondrion degradation; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; response to oxidative stress; phosphorylation

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset