Mouse PIK3R1 Monoclonal Antibody | anti-PIK3R1 antibody

PIK3R1 (Phosphoinositide-3-Kinase, Regulatory Subunit 1 (alpha), GRB1, p85, p85-ALPHA) (AP)

Cat. Num. AAA6164209

• Gene Names: PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
• Applications: Western Blot
• Purity: Purified
• Synonyms: PIK3R1; Monoclonal Antibody; PIK3R1 (Phosphoinositide-3-Kinase; Regulatory Subunit 1 (alpha); GRB1; p85; p85-ALPHA) (AP); Phosphoinositide-3-Kinase; p85-ALPHA; anti-PIK3R1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C10
• Specificity: Recognizes PIK3R1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 454

• Applicable Applications for anti-PIK3R1 antibody: Western Blot (WB), In situ Proximity Ligation Assay (Cell)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: PIK3R1 (AAH30815, 1aa-454aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MYNTVWNMEDLDLEYAKTDINCGTDLMFYIEMDPPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHKYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PIK3R1 monoclonal antibody (M02), clone 1C10. Western Blot analysis of PIK3R1 expression in Raw 264.7.)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between PDGFRA and PIK3R1 HeLa cells were stained with anti-PDGFRA rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Testing Data

(Detection limit for recombinant GST tagged PIK3R1 is 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-PIK3R1 antibody

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Product Categories/Family for anti-PIK3R1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 21410090
• NCBI GeneID: 5295
• NCBI Official Full Name: Phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 1
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; IMD36; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory

NCBI Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]