Mouse anti-Mouse, Rat PI3K p85 alpha Monoclonal Antibody | anti-PIK3R1 antibody

Anti-PI3K p85 alpha Antibody

Cat. Num. AAA8241502

• Gene Names: Pik3r1; PI3K; p50alpha; p55alpha; p85alpha
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: PI3K p85 alpha; Monoclonal Antibody; Anti-PI3K p85 alpha Antibody; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; anti-PIK3R1 antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Specificity: Recognizes endogenous levels of PI3K p85 alpha protein.
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
• Sequence Length: 724

• Applicable Applications for anti-PIK3R1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/100 - 1/200)
• Immunogen: Recombinant protein corresponding to mouse PI3K p85 alpha.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of PI3K p85 alpha expression in NIH3T3 (A), rat liver (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PI3K p85 alpha staining in rat liver,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-PIK3R1 antibody

Mouse monoclonal to PI3K p85 alpha

NCBI and Uniprot Product Information

• NCBI GI #: 341941221
• NCBI GeneID: 18708
• UniProt Accession #: P26450; Q8K3B3
• Molecular Weight: 83,517 Da
• NCBI Official Full Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• NCBI Official Symbol: Pik3r1
• NCBI Official Synonym Symbols: PI3K; p50alpha; p55alpha; p85alpha
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: Pik3r1
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
• UniProt Entry Name: P85A_MOUSE

Uniprot Description

PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Enzyme, regulatory subunit

Cellular Component: cis-Golgi network; cytoplasm; cytosol; intercellular junction; membrane; neuron projection; nucleus; phosphoinositide 3-kinase complex; plasma membrane; protein complex

Molecular Function: 1-phosphatidylinositol-3-kinase activity; 1-phosphatidylinositol-3-kinase regulator activity; ATPase binding; calmodulin binding; ErbB-3 class receptor binding; estrogen receptor binding; insulin binding; insulin receptor binding; insulin receptor substrate binding; insulin-like growth factor receptor binding; neurotrophin TRKA receptor binding; phosphoinositide 3-kinase regulator activity; phosphoprotein binding; platelet-derived growth factor receptor binding; protein binding; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; protein kinase binding; protein phosphatase binding; receptor binding; receptor tyrosine kinase binding; transcription factor binding; ubiquitin protein ligase binding

Biological Process: B cell differentiation; cell glucose homeostasis; cellular response to insulin stimulus; DNA damage response, signal transduction resulting in induction of apoptosis; glucose metabolic process; induction of apoptosis via death domain receptors; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; negative regulation of cell-matrix adhesion; negative regulation of heart rate; negative regulation of osteoclast differentiation; negative regulation of proteolysis; negative regulation of smooth muscle cell proliferation; NFAT protein import into nucleus; phosphoinositide 3-kinase cascade; positive regulation of cell migration; positive regulation of myoblast differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of RNA splicing; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; protein amino acid phosphorylation; protein stabilization; protein transport; regulation of phosphoinositide 3-kinase activity; regulation of stress fiber formation; response to cAMP; response to glucocorticoid stimulus; response to insulin stimulus; signal transduction; transport