Mouse anti-Human Phosphofructokinase Monoclonal Antibody | anti-PFKL antibody

Phosphofructokinase, Liver (PFKL, 6-phosphofructokinase Liver Type, DKFZp686G1648, DKFZp686L2097, FLJ30173, FLJ40909, Phosphofructo-1-kinase Isozyme B, PFK-B, Phosphofructokinase 1, Phosphohexokinase) (HRP)

Cat. Num. AAA6154138

• Gene Names: PFKL; PFK-B; PFK-L; ATP-PFK
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Phosphofructokinase; Monoclonal Antibody; Liver (PFKL; 6-phosphofructokinase Liver Type; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; Phosphofructo-1-kinase Isozyme B; PFK-B; Phosphofructokinase 1; Phosphohexokinase) (HRP); anti-PFKL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2A9
• Specificity: Recognizes human PFKL.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-PFKL antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa441-541 from human PFKL (NP_001002021) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: PKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALL
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.74kD).)

Western Blot (WB)

(PFKL monoclonal antibody. Western Blot analysis of PFKL expression in HeLa)

Product Categories/Family for anti-PFKL antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 590122057
• NCBI GeneID: 5211
• NCBI Accession #: NP_001002021
• NCBI GenBank Nucleotide #: NM_001002021
• UniProt Accession #: P17858; Q96A64; Q96IH4; Q9BR91
• Molecular Weight: 90,203 Da
• NCBI Official Full Name: ATP-dependent 6-phosphofructokinase, liver type isoform a
• NCBI Official Synonym Full Names: phosphofructokinase, liver
• NCBI Official Symbol: PFKL
• NCBI Official Synonym Symbols: PFK-B; PFK-L; ATP-PFK
• NCBI Protein Information: ATP-dependent 6-phosphofructokinase, liver type; 6-phosphofructokinase type B; ATP-PFK; liver-type 1-phosphofructokinase; phosphofructo-1-kinase isozyme B; phosphohexokinase
• UniProt Protein Name: 6-phosphofructokinase, liver type
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKL
• UniProt Synonym Gene Names: PFK-B
• UniProt Entry Name: K6PL_HUMAN

Uniprot Description

PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: 6-phosphofructokinase complex; membrane; cytosol

Molecular Function: identical protein binding; protein binding; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity

Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; response to glucose stimulus; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process; protein oligomerization; protein homotetramerization; negative regulation of insulin secretion