Mouse PDE5A Monoclonal Antibody | anti-PDE5A antibody

PDE5A (Phosphodiesterase 5A, cGMP-Specific, CGB-PDE, CN5A, PDE5, PDE5A1) (FITC)

Cat. Num. AAA6178350

• Gene Names: PDE5A; CN5A; PDE5; CGB-PDE
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified
• Synonyms: PDE5A; Monoclonal Antibody; PDE5A (Phosphodiesterase 5A; cGMP-Specific; CGB-PDE; CN5A; PDE5; PDE5A1) (FITC); Phosphodiesterase 5A; PDE5A1; anti-PDE5A antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 1F11
• Specificity: Recognizes PDE5A.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-PDE5A antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: PDE5A (NP_001074, 29aa-138aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: SVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTP
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDE5A monoclonal antibody (M02), clone 1F11. Western Blot analysis of PDE5A expression in Jurkat.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PDE5A on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PDE5A on HeLa cell. [antibody concentration 10 ug/ml])

Related Product Information for anti-PDE5A antibody

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq]

Product Categories/Family for anti-PDE5A antibody

Antibodies; Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 61744435
• NCBI GeneID: 8654
• NCBI Accession #: NP_001074
• NCBI GenBank Nucleotide #: NM_001083.3
• UniProt Accession #: O76074; O75026; O75887; Q86UI0; Q86V66; Q9Y6Z6; A0AV69; A8K2C4
• Molecular Weight: 94,805 Da
• NCBI Official Full Name: cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 5A
• NCBI Official Symbol: PDE5A
• NCBI Official Synonym Symbols: CN5A; PDE5; CGB-PDE
• NCBI Protein Information: cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Protein Name: cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE5A
• UniProt Synonym Gene Names: PDE5; CGB-PDE

NCBI Description

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: EC 3.1.4.35; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; cGMP binding

Biological Process: cGMP catabolic process