Mouse Phosphatase-1 Monoclonal Antibody | anti-PPP1R14A antibody

Mouse Anti Human Protein Phosphatase-1 Regulatory Subunit-14A

Cat. Num. AAA140305

• Gene Names: PPP1R14A; CPI17; CPI-17; PPP1INL
• Applications: ELISA, Western Blot
• Purity: PPP1R14A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Phosphatase-1; Monoclonal Antibody; Mouse Anti Human Protein Phosphatase-1 Regulatory Subunit-14A; PPP1R14A Antibody; Protein Phosphatase-1 Regulatory Subunit-14A; Mouse Anti Human; Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; CPI17; CPI-17; PPP1INL; PPP1R14A; anti-PPP1R14A antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chain and kappa light chain
• Clone Number: P4H10AT
• Purity/Purification: PPP1R14A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 120

• Applicable Applications for anti-PPP1R14A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: PPP1R14A antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.
• Immunogen: Anti-human PPP1R14A mAb is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPP1R14A amino acids 1-147 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-PPP1R14A antibody

Introduction: PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. Inhibition of PPP1R14A results to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. Human pregnancy is characterized by the increases of PKN1 expression and CPI-17 phosphorylation in the myometrium. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. PPP1R14A siRNA decreased the level of merlin phosphorylation and consequently Ras and ERK activity in human tumor cell lines. PKC/CPI-17 medi

Product Categories/Family for anti-PPP1R14A antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 345441807
• NCBI GeneID: 94274
• NCBI Accession #: NP_001230876.1
• NCBI GenBank Nucleotide #: NM_001243947.1
• Molecular Weight: 13,480 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 14A isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 14A
• NCBI Official Symbol: PPP1R14A
• NCBI Official Synonym Symbols: CPI17; CPI-17; PPP1INL
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17-kDa PKC-potentiated inhibitory protein of PP1; protein kinase C-potentiated inhibitor protein of 17 kDa
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 14A
• UniProt Gene Name: PPP1R14A
• UniProt Synonym Gene Names: CPI17; PPP1INL; CPI-17
• UniProt Entry Name: PP14A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A.

Protein type: Inhibitor; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm

Molecular Function: protein phosphatase inhibitor activity

Biological Process: regulation of phosphorylation; negative regulation of catalytic activity