Mouse anti-Human PGM3 Monoclonal Antibody | anti-PGM3 antibody

PGM3 (Phosphoacetylglucosamine Mutase, PAGM, Acetylglucosamine Phosphomutase, N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3, AGM1, DKFZp434B187) APC

Cat. Num. AAA6138205

• Gene Names: PGM3; AGM1; PAGM; IMD23; PGM 3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGM3; Monoclonal Antibody; PGM3 (Phosphoacetylglucosamine Mutase; PAGM; Acetylglucosamine Phosphomutase; N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3; AGM1; DKFZp434B187) APC; anti-PGM3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1E2-1B12
• Specificity: Recognizes human PGM3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PGM3 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC-P: 2ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-543 from human PGM3 (AAH01258) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (85.36kD).)

Western Blot (WB)

(PGM3 monoclonal antibody Western Blot analysis of PGM3 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody. Lane 1: PGM3 transfected lysate (59.9kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml])

Immunoprecipitation (IP)

(Immunoprecipitation of PGM3 transfected lysate using PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged PGM3 is ~3ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-PGM3 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12654830
• NCBI GeneID: 5238
• Molecular Weight: 62,941 Da
• NCBI Official Full Name: Homo sapiens phosphoglucomutase 3, mRNA
• NCBI Official Synonym Full Names: phosphoglucomutase 3
• NCBI Official Symbol: PGM3
• NCBI Official Synonym Symbols: AGM1; PAGM; IMD23; PGM 3
• NCBI Protein Information: phosphoacetylglucosamine mutase; N-acetylglucosamine-phosphate mutase 1; acetylglucosamine phosphomutase

NCBI Description

This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]