Mouse anti-Human Perforin Monoclonal Antibody | anti-PRF1 antibody

Mouse Anti Human Perforin: RPE

Cat. Num. AAA225827

• Gene Names: PRF1; P1; PFP; HPLH2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Synonyms: Perforin; Monoclonal Antibody; Mouse Anti Human Perforin: RPE; Perforin antibody; dG9; anti-PRF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: dG9
• Purity/Purification: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - Liquid
• Sequence Length: 555

• Applicable Applications for anti-PRF1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Max. Dilution: Neat; Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
• Immunogen: Purified granules from human YT lymphoma cell line
• Preservative Stabilizers: <0.1% Sodium Azide (NaN3) 0.2% Bovine Serum Albumin
• Buffer Solution: Phosphate Buffered Saline
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE.; This product should be stored undiluted.; This product is photosensitive and should be protected from light.

Testing Data

(Intracellular staining of perforin in human peripheral blood cells with Mouse anti Human perforin:RPE )

Testing Data

(Figure A. FITC conjugated Mouse anti Human CD56 and RPE conjugated mouse IgG2b isotype control . Figure B. FITC conjugated Mouse Anti Human CD56 and RPE conjugated Mouse anti Human Perforin . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, fixed and permeabilized. Gated on live, single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)

Related Product Information for anti-PRF1 antibody

Mouse anti Human perforin, clone dG9 recognizes perforin a 60-70 kDa glycoprotein largely expressed in CD8+ cytotoxin T lymphocytes and Natural Killer cells. It aids in defense against virus-infected or neoplastic cells by inducing apoptosis. Perforin achieves this by inserting a portion of its C-terminus region into the lipid membrane of the target in a calcium dependent manner. Subsequent polymerization with other perforin molecules forms a pore in the membrane. These pores allow the free influx and efflux of ions and proteins though the cell membrane. This disruption to homeostasis results in tonic shock which can ultimately lead to apoptosis and DNA degradation (Osinska et al. 2014).Genetic abnormalities to the perforin gene have been shown to be linked to abnormal immune system functioning, the development of immune diseases as well as lymphomas and leukemias (Osinska et al. 2014). This clone dG9 has been used in flow cytometry experiments to examine intracellular perforin in tumour specific CD8+ T cells incubated with autologous primary breast cancer cells and Tregs (Su et al. 2017).

NCBI and Uniprot Product Information

• NCBI GI #: 133908621
• NCBI GeneID: 5551
• NCBI Accession #: NP_001076585.1
• NCBI GenBank Nucleotide #: NP_001076585.1
• UniProt Accession #: P14222
• NCBI Official Full Name: perforin-1
• NCBI Official Synonym Full Names: perforin 1
• NCBI Official Symbol: PRF1
• NCBI Official Synonym Symbols: P1; PFP; HPLH2
• NCBI Protein Information: perforin-1
• UniProt Protein Name: Perforin-1
• Protein Family: Perforin
• UniProt Gene Name: PRF1
• UniProt Synonym Gene Names: PFP; P1; PFP
• UniProt Entry Name: PERF_HUMAN

NCBI Description

This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]

Uniprot Description

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: membrane; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; calcium ion binding; wide pore channel activity

Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; defense response to virus; transmembrane transport; protein homooligomerization

Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2