Rat anti-Mouse PECAM-1 Monoclonal Antibody | anti-PECAM-1 antibody
PECAM-1, Mouse, mAb MEC7.46, biotinylated
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Synonyms
PECAM-1; Monoclonal Antibody; Mouse; mAb MEC7.46; biotinylated; CD31; platelet-endothelial cell adhesion molecule-1; Gene name: Pecam1; PECAM-1 Mouse mAb MEC7.46 Biotin 50ug; anti-PECAM-1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1
Form/Format
0.5ml (100ug/ml) 0.2um filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Sequence Length
738
Applicable Applications for anti-PECAM-1 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin
Application Notes
The typical starting working dilution is 1:50.
FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1)
IHC-F: Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. As (Ref.1).
IHC-P: Serial paraffin-embedded 4um sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium (ref. 3).
IP: Lysate of 35S-Methionine labeled 5HV cells was used. Molecule of ~130 kDa.
FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1)
IHC-F: Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. As (Ref.1).
IHC-P: Serial paraffin-embedded 4um sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium (ref. 3).
IP: Lysate of 35S-Methionine labeled 5HV cells was used. Molecule of ~130 kDa.
Immunogen
Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)
Conjugation
Biotin
Positive Control
Mouse t-end.1 cells
Negative Control
L929 cells (Fibroblasts)
Preparation and Storage
Product should be stored at 4 degree C.
Under recommended storage conditions, product is stable for one year.
Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-PECAM-1 antibody
The monoclonal antibody MEC7.46 recognizes the mouse form of the platelet-endothelial cell adhesion molecule (PECAM)-1 (CD31). PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. PECAM-1 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1. The reactivity of the monoclonal antibody MEC7.46 is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cells of all mouse blood vessels both in normal, inflamed and tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1. Staining of MEC7.46 can be seen on capillaries, veins, arteries and liver sinusoids.
Product Categories/Family for anti-PECAM-1 antibody
References
1. Vecchi, A et al; Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur J Cell Biol 1994, 63: 247
2. Kusters, B et al. Vascular Endothelial Growth Factor-A165 Induces Progression of Melanoma Brain Metastases without Induction of Sprouting Angiogenesis. Cancer Res 2002, 62: 341
3. Clarijs, R et al; Lymphatics and lymphatic-like structures in melanoma. Thesis 2003
4. Xu, Q et al; Circulating progenitor cells regenerate endothelium of vein graft atherosclerosis, which is diminished in ApoE-deficient mice. Circ Res 2003, 93: e76
5. Smetsers, T et al; Localization and characterization of melanoma-associated glycosaminoglycans: Differential expression of chondroitin and heparan sulfate epitopes in melanoma. Canc Res 2003, 63: 2965
2. Kusters, B et al. Vascular Endothelial Growth Factor-A165 Induces Progression of Melanoma Brain Metastases without Induction of Sprouting Angiogenesis. Cancer Res 2002, 62: 341
3. Clarijs, R et al; Lymphatics and lymphatic-like structures in melanoma. Thesis 2003
4. Xu, Q et al; Circulating progenitor cells regenerate endothelium of vein graft atherosclerosis, which is diminished in ApoE-deficient mice. Circ Res 2003, 93: e76
5. Smetsers, T et al; Localization and characterization of melanoma-associated glycosaminoglycans: Differential expression of chondroitin and heparan sulfate epitopes in melanoma. Canc Res 2003, 63: 2965
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet and endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
NCBI Protein Information
platelet endothelial cell adhesion molecule
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
UniProt Entry Name
PECA1_HUMAN
NCBI Description
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Uniprot Description
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q23.3
Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction
Molecular Function: protein binding
Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis
Research Articles on PECAM-1
Similar Products
Product Notes
The PECAM-1 pecam1 (Catalog #AAA584848) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The PECAM-1, Mouse, mAb MEC7.46, biotinylated reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PECAM-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin. The typical starting working dilution is 1:50. FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1) IHC-F: Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. As (Ref.1). IHC-P: Serial paraffin-embedded 4um sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium (ref. 3). IP: Lysate of 35S-Methionine labeled 5HV cells was used. Molecule of ~130 kDa. Researchers should empirically determine the suitability of the PECAM-1 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PECAM-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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