Human anti-Human PD1 Monoclonal Antibody | anti-PD1 antibody

PD1 Monoclonal Antibody (Humanized)

Cat. Num. AAA3000291

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human
• Applications: Neutralization Assay
• Purity: Recombinant IgG antibodies were expressed and secreted by 293F cells and purified by Protein A.
• Synonyms: PD1; Monoclonal Antibody; PD1 Monoclonal Antibody (Humanized); Programmed cell death protein 1; Protein PD-1; hPD-1; CD279; PDCD1; anti-PD1 antibody

• Host: Human
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: PD1 monoclonal antibody (Humanized) detects endogenous levels of human PD1 protein. Variable region genes (including VH &VL) of this antibody were derived from a single B cell by PCR.
• Purity/Purification: Recombinant IgG antibodies were expressed and secreted by 293F cells and purified by Protein A.
• Form/Format: 1mg/ml in PBS with 50% Glycerol.
• Sequence Length: 288

• Applicable Applications for anti-PD1 antibody: Neutralization, Block
• Immunogen: Variable region genes (including VH &VL) of this antibody were derived from a single B cell by PCR.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot shows binding reaction of PD1 monoclonal antibody (Humanized) and recombinant human PD1 (with Fc tag). Recombinant human PD1 (Fc) was electrophoresed by SDS-PAGE and transferred on PVDF membrane. PVDF membrane was probed with PD1 monoclonal antibody (Humanized) followed by HRP-conjugated Anti-Human IgG Fab secondary Antibody.)

Related Product Information for anti-PD1 antibody

The humanized monoclonal anti-PD1 antibody can effectively block the combination between PDL1 and PD1, and will not cause HAMA reaction (anti mouse antibody response). Programmed cell death protein 1 (PD-1) (UniProt Q15116), is a type I single transmembrane protein encoded by the PDCD1 gene in human. T-cell activation is generally self-limited as activated T cells express receptors such as PD-1. Maintenance of peripheral tolerance by preventing the development of autoimmunity due to T lymphocytes specific for self-antigens is essential for homeostasis of the immune system. PD-1 can bind two different but related ligands, PDL-1 and PDL-2.PD-1 receptor and its ligands PD-L1 and PD-L2play a vital role in the induction and maintenance of peripheral tolerance. The humanized monoclonal anti-PD1 antibody has demonstrated durable antitumor activity.

NCBI and Uniprot Product Information

• NCBI GI #: 145559515
• NCBI GeneID: 5133
• UniProt Accession #: Q15116
• Molecular Weight: ~ 32kDa
• NCBI Official Full Name: Programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.