Human HLA-E ELISA Kit | HLA-E elisa kit

Human HLA-E ELISA Kit

Cat. Num. AAA3803319

• Gene Names: HLA-E; QA1; HLA-6.2
• Reactivity: Human
• Synonyms: HLA-E; Human HLA-E ELISA Kit; HLA-E elisa kit

• Reactivity: Human
• Sequence Length: 358

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 125 pg/ml-2000 pg/ml
• Sensitivity: 10 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HLA-E elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHLA-Ein the sample, This HLA-EELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHLA-Econcentration. The concentration ofHLA-Ein the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62912479
• NCBI GeneID: 3133
• NCBI Accession #: NP_005507
• NCBI GenBank Nucleotide #: NP_005507.3
• UniProt Accession #: P13747
• NCBI Official Full Name: HLA class I histocompatibility antigen, alpha chain E
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, E
• NCBI Official Symbol: HLA-E
• NCBI Official Synonym Symbols: QA1; HLA-6.2
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain E
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain E
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-E
• UniProt Synonym Gene Names: HLA-6.2; HLAE
• UniProt Entry Name: HLAE_HUMAN

NCBI Description

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-E: Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. Belongs to the MHC class I family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; early endosome membrane; plasma membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; MHC class I protein binding; beta-2-microglobulin binding; receptor binding

Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; positive regulation of natural killer cell mediated immunity; regulation of natural killer cell mediated immunity; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; antigen processing and presentation of endogenous peptide antigen via MHC class Ib; protection from natural killer cell mediated cytotoxicity