Mouse anti-Canine PD-L1 Monoclonal Antibody | anti-PD-L1 antibody
Canine PD-L1 Monoclonal Antibody
Reactivity
Canine
Applications
Flow Cytometry, Functional Assay
Synonyms
PD-L1; Monoclonal Antibody; Canine PD-L1 Monoclonal Antibody; anti-PD-L1 antibody
Host
Mouse
Reactivity
Canine
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody recognizes canine PD-L1.
Form/Format
Protein G-purified antibody in PBS, pH7.4. Concentration is lot-specific; please refer to the vial label.
Applicable Applications for anti-PD-L1 antibody
Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry: Detection of PD-L1 expression on CLGL-90 cells (Chronic Large Granular Lymphocytic-90) stained with JC071 (dark gray) vs. mouse IgA isotype control (light gray).
Endusers should determine optimal antibody concentrations for their applications.
Inhibition of PD-1 and PD-L1 Interaction
JC071 antibody added to ELISA plates coated with PD-L1 followed by the addition of biotinylated PD-1 and streptavidin-HRP. JC071 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of culture medium or PBS.
Endusers should determine optimal antibody concentrations for their applications.
Inhibition of PD-1 and PD-L1 Interaction
JC071 antibody added to ELISA plates coated with PD-L1 followed by the addition of biotinylated PD-1 and streptavidin-HRP. JC071 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of culture medium or PBS.
Antigen
Recombinant extracellular domain of canine PD-L1 fused with the Fc domain of human IgG1 expressed in D. melanogaster S2 cells.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C to-70 degree C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Related Product Information for anti-PD-L1 antibody
Background: Each year, thousands of dogs are identified with cancer. Canine cancers bear many of the hallmarks of human cancers including tissue location, tumor progression, and response to chemotherapy and radiation. Unfortunately, canine lymphocyte populations are not as well defined as human lymphocytes, and reagents that target immune checkpoint pathways are not yet widely available for veterinary research. Interruption of the programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) pathway is an established and effective therapeutic strategy in human oncology and may be effective for treating canine cancers as well.
Product Categories/Family for anti-PD-L1 antibody
References
Choi JW et al. 2020 Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function. PLoS One. 2020; 15(7): e0235518.
Similar Products
Product Notes
The PD-L1 (Catalog #AAA191624) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Canine PD-L1 Monoclonal Antibody reacts with Canine and may cross-react with other species as described in the data sheet. AAA Biotech's PD-L1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow Cytometry: Detection of PD-L1 expression on CLGL-90 cells (Chronic Large Granular Lymphocytic-90) stained with JC071 (dark gray) vs. mouse IgA isotype control (light gray). Endusers should determine optimal antibody concentrations for their applications. Inhibition of PD-1 and PD-L1 Interaction JC071 antibody added to ELISA plates coated with PD-L1 followed by the addition of biotinylated PD-1 and streptavidin-HRP. JC071 antibody blocked binding of PD-1 to PD-L1 as compared with binding of PD-1 to PD-L1 in the presence of culture medium or PBS. Researchers should empirically determine the suitability of the PD-L1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-L1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
