Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of PCBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PCBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). )
Mouse anti-Human PCBP1 Monoclonal Antibody | anti-PCBP1 antibody
PCBP1
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of PCBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PCBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). )
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of PCBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PCBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: K562 cell lysate Lane 4.: 293T cell lysateLane 5.: Jurkat cell lysate Lane 6.: MCF7 cell lysate )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: K562 cell lysate Lane 4.: 293T cell lysateLane 5.: Jurkat cell lysate Lane 6.: MCF7 cell lysate )
NCBI and Uniprot Product Information
NCBI Description
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]
Uniprot Description
hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
Protein type: RNA splicing; Translation; RNA-binding
Chromosomal Location of Human Ortholog: 2p13-p12
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; cytoplasm; ribonucleoprotein complex
Molecular Function: protein binding; RNA binding; single-stranded DNA binding
Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
Research Articles on PCBP1
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Product Notes
The PCBP1 pcbp1 (Catalog #AAA201224) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PCBP1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCBP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Researchers should empirically determine the suitability of the PCBP1 pcbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCBP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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