Western Blot (WB)
(Fig-1: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 0.5 ug/ml on recombinant and 2 ug/ml on Raji lysate.)
Mouse anti-Human PAX8 Monoclonal Antibody | anti-PAX8 antibody
Monoclonal Antibody to PAX8 (Clone: ABM5F33)
Protein G Chromatography
Protein G Chromatography
Flow Cytometry: 0.5-1 ug/10^6
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig-1: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 0.5 ug/ml on recombinant and 2 ug/ml on Raji lysate.)
Western Blot (WB)
(Fig-1: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 0.5 ug/ml on recombinant and 2 ug/ml on Raji lysate.)
Flow Cytometry (FC/FACS)
(Fig-2: Intracellular flow analysis of Pax8 in Raji cells using 0.5 ug/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS)
(Fig-2: Intracellular flow analysis of Pax8 in Raji cells using 0.5 ug/10^6 cells of antibody (Clone: ABM5F33). Green represents isotype control; red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
Uniprot Description
PAX8: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Interacts with WWTR1. Expressed in the excretory system, thyroid gland and Wilms tumors. 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 2q14.1
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; protein binding; thyroid-stimulating hormone receptor activity; transcription factor activity
Biological Process: anatomical structure morphogenesis; central nervous system development; inner ear morphogenesis; kidney development; mesonephros development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; pronephros development; regulation of apoptosis; thyroid gland development; transcription, DNA-dependent; ureteric bud branching; urogenital system development
Disease: Hypothyroidism, Congenital, Nongoitrous, 2
Research Articles on PAX8
Similar Products
Product Notes
The PAX8 pax8 (Catalog #AAA668451) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to PAX8 (Clone: ABM5F33) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAX8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). WB: 2-4 ug/ml Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the PAX8 pax8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
