Mouse PAX8 Monoclonal Antibody | anti-PAX8 antibody

Anti-PAX8 Antibody (clone 1F8-3A8)

Cat. Num. AAA2401585

• Reactivity: Human; Predicted: Mouse, Primate
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Protein A purified
• Synonyms: PAX8; Monoclonal Antibody; Anti-PAX8 Antibody (clone 1F8-3A8); Goat Polyclonal to Human PAX8; Paired box 8; Paired domain gene 8; Paired box gene 8; Paired box protein Pax-8; anti-PAX8 antibody

• Host: Mouse
• Reactivity: Human; Predicted: Mouse, Primate
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1F8-3A8
• Specificity: Western blot analysis detects a prominent ~48 kD protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 1 mg/ml BSA, 0.05% sodium azide, 30% glycerol.
• Sequence Length: 450

• Applicable Applications for anti-PAX8 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:50-1:200; IHC-P: 10 ug/ml; WB: 1:500-1:2000
• Immunogen: Purified internal fragment of human recombinant PAX8 expressed in E. coli.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(IF using PAX8 Antibody (1F8-3A8))

Western Blot (WB)

(WB using PAX8 Antibody (1F8-3A8))

Western Blot (WB)

(WB using PAX8 Antibody (1F8-3A8))

Western Blot (WB)

(WB using PAX8 Antibody (1F8-3A8))

Related Product Information for anti-PAX8 antibody

PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins thatcontain a paired box domain, an octapeptide, and a paired-typehomeodomain. This nuclear protein is involved in thyroid follicular celldevelopment and expression of thyroid-specific genes. Mutations in thisgene have been associated with thyroid dysgenesis, thyroid follicularcarcinomas and atypical follicular thyroid adenomas.

NCBI and Uniprot Product Information

• NCBI GI #: 5803117
• NCBI GeneID: 7849
• NCBI Accession #: NP_003457.1
• NCBI GenBank Nucleotide #: NM_003466.3
• UniProt Accession #: Q06710; Q09155; Q16337; Q16338; Q16339; Q4ZG35; Q96J49
• Molecular Weight: 31,044 Da
• NCBI Official Full Name: paired box protein Pax-8 isoform PAX8A
• NCBI Official Synonym Full Names: paired box 8
• NCBI Official Symbol: PAX8
• NCBI Protein Information: paired box protein Pax-8
• UniProt Protein Name: Paired box protein Pax-8
• Protein Family: Paired box protein
• UniProt Gene Name: PAX8

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.