Western Blot (WB)
(Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (SMC-563). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (SMC-563) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
Mouse anti-Rat, Mouse Parvalbumin Monoclonal Antibody | anti-Pvalb antibody
Paravalbumin Antibody, Clone C12: ATTO 594
Gene Names
Pvalb; Pva; PALB1
Reactivity
Rat, Mouse
Applications
Western Blot, ELISA
Purity
Protein A Purified
Synonyms
Parvalbumin; Monoclonal Antibody; Paravalbumin Antibody; Clone C12: ATTO 594; Mouse Anti-Rat Paravalbumin Monoclonal IgG2a; Parvalbumin alpha antibody; PRVA_HUMAN antibody; PVALB antibody; anti-Pvalb antibody
Host
Mouse
Reactivity
Rat, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
C12
Specificity
Detects 12 kDa.
Purity/Purification
Protein A Purified
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1mg/ml (varies by lot)
Sequence Length
110
Applicable Applications for anti-Pvalb antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500
Immunogen
Synthetic peptide from the full length Rat Parvalbumin protein
Immunogen Species
Rat
Tissue Specificity
Retina. Expressed throughout the horizontal cells and/or terminals.
Conjugate
ATTO 594
Cellular Localization
Cytoplasmic vesicle membrane
Certificate of Analysis
A 1:500 dilution was sufficient for detection of Parvalbumin in 15 ug of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Preparation and Storage
Store at -20 degree C
Shipping Temperature: Blue Ice or 4 degree C
Shipping Temperature: Blue Ice or 4 degree C
Western Blot (WB)
(Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (SMC-563). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (SMC-563) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
Western Blot (WB)
(Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12 (SMC-563). Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody (SMC-563) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
Related Product Information for anti-Pvalb antibody
Background: Parvalbumin is involved in calcium signaling. Alterations in the function of parvalbumin-expressing neurons hav been implicated in Alzheimer's, autism, schizophrenia, age-related cognitive defects and some forms of cancer (1).
Product Categories/Family for anti-Pvalb antibody
References
1. Cates. M.S., Teodoro M.L., Phillips G.N. (2002) Biophys J. 82(3): 1133-1146.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
parvalbumin alpha
NCBI Official Synonym Full Names
parvalbumin
NCBI Official Symbol
Pvalb
NCBI Official Synonym Symbols
Pva; PALB1
NCBI Protein Information
parvalbumin alpha
UniProt Protein Name
Parvalbumin alpha
Protein Family
UniProt Gene Name
Pvalb
UniProt Synonym Gene Names
Pva
UniProt Entry Name
PRVA_RAT
NCBI Description
binds two calcium ions; may have evolved from an ancestral four domain calcium binding protein [RGD, Feb 2006]
Uniprot Description
PVA: In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Belongs to the parvalbumin family.
Cellular Component: protein complex; cell soma; axon; cytoplasm; terminal button; nucleus
Molecular Function: protein homodimerization activity; protein heterodimerization activity; calcium ion binding
Biological Process: cytosolic calcium ion homeostasis
Research Articles on Pvalb
Similar Products
Product Notes
The Pvalb pvalb (Catalog #AAA8005809) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Paravalbumin Antibody, Clone C12: ATTO 594 reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Parvalbumin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500. Researchers should empirically determine the suitability of the Pvalb pvalb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parvalbumin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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