Mouse anti-Human PAR4 Monoclonal Antibody | anti-PAR4 antibody

PAR4 (Proteinase Activated Receptor 4, PAR-4, Coagulation Factor II Receptor-like 3, F2RL3, Thrombin Receptor-like 3) (MaxLight 405)

Cat. Num. AAA6191626

• Gene Names: F2RL3; PAR4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PAR4; Monoclonal Antibody; PAR4 (Proteinase Activated Receptor 4; PAR-4; Coagulation Factor II Receptor-like 3; F2RL3; Thrombin Receptor-like 3) (MaxLight 405); anti-PAR4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5F4
• Specificity: Recognizes human F2RL3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
• Sequence Length: 3334

• Applicable Applications for anti-PAR4 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa18-83 from F2RL3 (NP_003941) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: GGTQTPSVYDESGSTGGGDDSTPSILPAPRGYPGQVCANDSDTLELPDSSRALLLGWVPTRLVPA
• Conjugate: MaxLight405
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-PAR4 antibody

Proteinase-Activated Receptor 4 (PAR4) is a member of the Proteinase-Activated Receptor subfamily. Along with other protease-activated receptors, PAR4 mediates the effects of the enzyme, thrombin which include activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of the receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR4 expression has been documented in the periphery, primarily in blood platelets.

Product Categories/Family for anti-PAR4 antibody

Antibodies; Receptors

References

1. Polymorphism in the protease activated receptor-4 gene region associates with platelet activation and perioperative myocardial injury. Muehlschlegel JD, Perry TE, Liu KY, Fox AA, Smith S, Lichtner P, Collard CD, Shernan SK, Hartwig JH, Body SC, Hoffmeister KM.American Journal of Hematology DOI: 10.1002/ ajh.22244

NCBI and Uniprot Product Information

• NCBI GI #: 1653962240
• NCBI GeneID: 9002
• NCBI Accession #: NM_003950.4
• NCBI GenBank Nucleotide #: NM_003950
• UniProt Accession #: Q96RI0
• NCBI Official Full Name: Homo sapiens F2R like thrombin or trypsin receptor 3 (F2RL3), mRNA
• NCBI Official Synonym Full Names: F2R like thrombin or trypsin receptor 3
• NCBI Official Symbol: F2RL3
• NCBI Official Synonym Symbols: PAR4
• NCBI Protein Information: proteinase-activated receptor 4
• UniProt Protein Name: Proteinase-activated receptor 4
• UniProt Gene Name: F2RL3
• UniProt Synonym Gene Names: PAR4; PAR-4
• UniProt Entry Name: PAR4_HUMAN

NCBI Description

This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016]

Uniprot Description

PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: thrombin receptor activity

Biological Process: platelet activation; platelet dense granule organization and biogenesis; response to wounding; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; signal transduction