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Western Blot (WB) (Western Blot detection against Immunogen (38.1kD).)

Mouse anti-Human PAPD7 Monoclonal Antibody | anti-PAPD7 antibody

PAPD7 (POLS, DNA Polymerase sigma, DNA Polymerase kappa, LAK-1, PAP-associated Domain-containing Protein 7, Terminal Uridylyltransferase 5, TUTase 5, Topoisomerase-related Function Protein 4-1, TRF4-1, TRF4, TUTASE5) APC

Gene Names
TENT4A; LAK1; POLK; POLS; TRF4; LAK-1; PAPD7; TRF41; TRF4-1; TUTASE5
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PAPD7; Monoclonal Antibody; PAPD7 (POLS; DNA Polymerase sigma; DNA Polymerase kappa; LAK-1; PAP-associated Domain-containing Protein 7; Terminal Uridylyltransferase 5; TUTase 5; Topoisomerase-related Function Protein 4-1; TRF4-1; TRF4; TUTASE5) APC; anti-PAPD7 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2F8
Specificity
Recognizes human POLS.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PAPD7 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa2-111 from human POLS (NP_008930) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETG
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (38.1kD).)

Western Blot (WB) (Western Blot detection against Immunogen (38.1kD).)

Testing Data

(Detection limit for recombinant GST tagged POLS is ~0.3ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged POLS is ~0.3ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of POLS over-expressed 293 cell line, cotransfected with POLS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of POLS over-expressed 293 cell line, cotransfected with POLS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Related Product Information for anti-PAPD7 antibody
The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described.
Product Categories/Family for anti-PAPD7 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60kDa
NCBI Official Full Name
terminal nucleotidyltransferase 4A isoform 1
NCBI Official Synonym Full Names
terminal nucleotidyltransferase 4A
NCBI Official Symbol
TENT4A
NCBI Official Synonym Symbols
LAK1; POLK; POLS; TRF4; LAK-1; PAPD7; TRF41; TRF4-1; TUTASE5
NCBI Protein Information
terminal nucleotidyltransferase 4A; non-canonical poly(A) RNA polymerase PAPD7
UniProt Protein Name
Non-canonical poly(A) RNA polymerase PAPD7
UniProt Gene Name
PAPD7
UniProt Synonym Gene Names
POLS; TRF4; TUTase 5; TRF4-1
UniProt Entry Name
PAPD7_HUMAN

NCBI Description

The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

POLS: DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Does not play a role in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family.

Protein type: EC 2.7.7.19; DNA repair, damage; DNA replication; Transferase

Chromosomal Location of Human Ortholog: 5p15

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: polynucleotide adenylyltransferase activity; DNA binding; SMC protein binding; metal ion binding; DNA-directed DNA polymerase activity

Biological Process: response to drug; cell division; mitotic chromosome condensation; double-strand break repair; sister chromatid cohesion

Research Articles on PAPD7

Similar Products

Product Notes

The PAPD7 papd7 (Catalog #AAA6138360) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PAPD7 (POLS, DNA Polymerase sigma, DNA Polymerase kappa, LAK-1, PAP-associated Domain-containing Protein 7, Terminal Uridylyltransferase 5, TUTase 5, Topoisomerase-related Function Protein 4-1, TRF4-1, TRF4, TUTASE5) APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAPD7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PAPD7 papd7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAPD7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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