Rat anti-Mouse Paired Immunoglobulin-like Receptor B Monoclonal Antibody | anti-PILRA antibody

Paired Immunoglobulin-like Receptor B (PIR-B)

Cat. Num. AAA601864

• Gene Names: PILRA; FDF03
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Paired Immunoglobulin-like Receptor B; Monoclonal Antibody; Paired Immunoglobulin-like Receptor B (PIR-B); Anti -Paired Immunoglobulin-like Receptor B (PIR-B); anti-PILRA antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7H89
• Specificity: Recognizes mouse Paired Immunoglobulin-like Receptor B (PIR-B). Does not crossreact with mouse PIR-A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose.

• Applicable Applications for anti-PILRA antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, ELISA and Western Blot.; Dilution: Western Blot: 1-2ug/ml. The detection limit is ~10ng/lane under non-reducing conditions. Reducing conditions not recommended.; Flow Cytometry: 25ug/ml. 10ul labels 1-2.5 x 10e5 cells in a total reaction volume 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome.; Direct ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well.
• Immunogen: NS0-derived recombinant mouse PIR-B (aa24-635, Accession #AAH26937).
• Hybridoma: Myeloma cells with spleen cells from Balb/c mice with NS0-derived, recombinant mouse Paired Ig-like Receptor B
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PILRA antibody

Mouse PIR-B is a 125kD type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% aa sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Product Categories/Family for anti-PILRA antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 30179909
• NCBI GeneID: 29992
• NCBI Accession #: NP_038467.2
• NCBI GenBank Nucleotide #: NM_013439.2
• UniProt Accession #: Q9UKJ1; Q8NHI1
• Molecular Weight: 34,005 Da
• NCBI Official Full Name: paired immunoglobulin-like type 2 receptor alpha isoform 1
• NCBI Official Synonym Full Names: paired immunoglobin-like type 2 receptor alpha
• NCBI Official Symbol: PILRA
• NCBI Official Synonym Symbols: FDF03
• NCBI Protein Information: paired immunoglobulin-like type 2 receptor alpha; cell surface receptor FDF03; inhibitory receptor PILR-alpha
• UniProt Protein Name: Paired immunoglobulin-like type 2 receptor alpha
• Protein Family: Paired immunoglobulin-like type 2 receptor
• UniProt Gene Name: PILRA
• UniProt Entry Name: PILRA_HUMAN

NCBI Description

Cell signaling pathways rely on a dynamic interaction between activating and inhibiting processes. SHP-1-mediated dephosphorylation of protein tyrosine residues is central to the regulation of several cell signaling pathways. Two types of inhibitory receptor superfamily members are immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptors and their non-ITIM-bearing, activating counterparts. Control of cell signaling via SHP-1 is thought to occur through a balance between PILRalpha-mediated inhibition and PILRbeta-mediated activation. These paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This particular gene encodes the ITIM-bearing member of the receptor pair, which functions in the inhibitory role. Alternative splicing has been observed at this locus and three variants, each encoding a distinct isoform, are described. [provided by RefSeq, Jul 2008]

Uniprot Description

PILRA: Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILRA is thought to act as a cellular signaling inhibitory receptor by recruiting cytoplasmic phosphatases like PTPN6/SHP-1 and PTPN11/SHP-2 via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Receptor for PIANP. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: viral reproduction; signal transduction