Testing Data
(MG-63 cells were stained with MBS602913 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.)
Mouse anti-Human mGluR5 Monoclonal Antibody | anti-Grm5 antibody
mGluR5 (Metabotropic Glutamate Receptor)
Gene Names
Grm5; mGluR5; mGlur5
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
mGluR5; Monoclonal Antibody; mGluR5 (Metabotropic Glutamate Receptor); Anti -mGluR5 (Metabotropic Glutamate Receptor); anti-Grm5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8H205
Specificity
Recognizes human mGluR5. Does not crossreact with reecombinant human mGluR1, R2, R3, R4, R7 or R8.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-Grm5 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, ELISA and Western Blot.
Dilution: Intracellular Flow Cytometry: 25ug/ml; Use 10ul to label 1-5x10e5 cells in a total reaction volume not exceeding 200ul. Cells must be fixed and permeablized using 4% paraformaldehyde and 0.1% saponin in PBS.
Direct ELISA: 0.5-1.0ug/ml; The detection limit for recombinant human mGluR5 is ~2ng/well
Western Blot: 1-2ug/ml; The detection limit for recombiant human mGluR5 is ~25ng/lane under non-reducing and reducing conditions.
Dilution: Intracellular Flow Cytometry: 25ug/ml; Use 10ul to label 1-5x10e5 cells in a total reaction volume not exceeding 200ul. Cells must be fixed and permeablized using 4% paraformaldehyde and 0.1% saponin in PBS.
Direct ELISA: 0.5-1.0ug/ml; The detection limit for recombinant human mGluR5 is ~2ng/well
Western Blot: 1-2ug/ml; The detection limit for recombiant human mGluR5 is ~25ng/lane under non-reducing and reducing conditions.
Immunogen
Recombinant protein corresponding to aa19-509 from human mGluR5, expressed in NSO cells (P41594).
Positive Control
Flow Cytometry: MG-63 cells
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(MG-63 cells were stained with MBS602913 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.)
Testing Data
(MG-63 cells were stained with MBS602913 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.)
Related Product Information for anti-Grm5 antibody
Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150kD, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212aa in length and contains an extracellular domain (ECD) of 558aa. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32aa deletion between aa877-908 in the cytoplasmic tail. Over aa19-509, human mGluR5 is 98% identical to mouse, rat and canine mGluR5.
Product Categories/Family for anti-Grm5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
131,885 Da
NCBI Official Full Name
Metabotropic glutamate receptor 5
NCBI Official Synonym Full Names
glutamate receptor, metabotropic 5
NCBI Official Symbol
Grm5
NCBI Official Synonym Symbols
mGluR5; mGlur5
NCBI Protein Information
metabotropic glutamate receptor 5; metabotropic glutamate receptor 5b; metabotropic glutamate receptor (mGluR5)
UniProt Protein Name
Metabotropic glutamate receptor 5
Protein Family
UniProt Gene Name
Grm5
UniProt Synonym Gene Names
Gprc1e; Mglur5
UniProt Entry Name
GRM5_RAT
NCBI Description
may play a role in synaptic transmission; may interact with mGluR1, another group I metabotropic glutamate receptor [RGD]
Uniprot Description
Function: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
Subunit structure: The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3
By similarity. Interacts with SIAH1 and GRASP. Interacts with NCDN. Ref.3 Ref.4 Ref.5 Ref.7
Subcellular location: Cell membrane; Multi-pass membrane protein.
Tissue specificity: Widely distributed in neuronal cells of the central nervous system.
Miscellaneous: Activated by quisqualate > glutamate > ibotenate > trans-1- aminocyclopentyl-1,3-dicarboxylate.
Sequence similarities: Belongs to the G-protein coupled receptor 3 family.
Research Articles on Grm5
Similar Products
Product Notes
The Grm5 grm5 (Catalog #AAA602913) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The mGluR5 (Metabotropic Glutamate Receptor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's mGluR5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, ELISA and Western Blot. Dilution: Intracellular Flow Cytometry: 25ug/ml; Use 10ul to label 1-5x10e5 cells in a total reaction volume not exceeding 200ul. Cells must be fixed and permeablized using 4% paraformaldehyde and 0.1% saponin in PBS. Direct ELISA: 0.5-1.0ug/ml; The detection limit for recombinant human mGluR5 is ~2ng/well Western Blot: 1-2ug/ml; The detection limit for recombiant human mGluR5 is ~25ng/lane under non-reducing and reducing conditions. Researchers should empirically determine the suitability of the Grm5 grm5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mGluR5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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