Rabbit anti-Human, Mouse p73 Monoclonal Antibody | anti-p73 antibody

p73 Antibody

Cat. Num. AAA9435720

• Gene Names: TP73; P73
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: p73; Monoclonal Antibody; p73 Antibody; p53 like transcription factor antibody; p53 related protein antibody; p53-like transcription factor antibody; p53-related protein antibody; p73 antibody; P73_HUMAN antibody; TP73 antibody; Tumor protein p73 antibody; anti-p73 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: ST05-76
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-p73 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of p73 on Hela cell lysates using anti-p73 antibody at 1/1, 000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-p73 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining p73 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-p73 antibody

The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents. The p73 isoform p73alpha inhibits drug-induced apoptosis in small cell lung carcinoma cells, while the p73 isoform p73beta promotes it. p73alpha also prevents Bax activation, mitochondrial dysfunction, caspase activation and is able to reduce apoptosis induced by the BH3-only protein PUMA (p53 upregulated modulator of apoptosis). There is an equilibrium between p73alpha and p73beta , demonstrated by the fact that p73alpha inhibits the pro-apoptotic effect of p73beta .

Product Categories/Family for anti-p73 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 187828902
• NCBI GeneID: 7161
• NCBI Accession #: NP_001119712.1
• NCBI GenBank Nucleotide #: NM_001126240.2
• UniProt Accession #: O15350; O15351; Q17RN8; Q5TBV5; Q5TBV6; Q8NHW9; Q8TDY5; Q8TDY6; Q9NTK8; B7Z7J4; B7Z9C1
• Molecular Weight: 69,623 Da
• NCBI Official Full Name: tumor protein p73 isoform b
• NCBI Official Synonym Full Names: tumor protein p73
• NCBI Official Symbol: TP73
• NCBI Official Synonym Symbols: P73
• NCBI Protein Information: tumor protein p73; p53-related protein; p53-like transcription factor
• UniProt Protein Name: Tumor protein p73
• UniProt Gene Name: TP73
• UniProt Synonym Gene Names: P73
• UniProt Entry Name: P73_HUMAN

NCBI Description

This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011]

Uniprot Description

p73: a protein of the p53 family of proteins. p73, like p53, may have tumor suppressor activity, but its multiple isoforms possess different and sometimes opposing functions. Participates in the apoptotic response to DNA damage. Phosphorylated in a cell cycle-dependent manner and negatively regulated by CDKs. When overproduced, activates transcription from p53-responsive promoters and induces apoptosis. Nine transactivation competent (TA) or dominant negative (DN) isoforms, derived from alternative-splicing and alternative promoters, have been described. NH2-terminally truncated p73 isoforms (DNp73) may be oncogenic. DNp73 is elevated in a number of cancers and is associated with adverse clinical outcomes and resistance to chemotherapy. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9; forms complex with p53 and CABLES1. Isoform Beta interacts with WWOX; interacts with HECW2.

Protein type: Tumor suppressor; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: nucleoplasm; Golgi apparatus; transcription factor complex; intracellular membrane-bound organelle; cell junction; chromatin; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; p53 binding; metal ion binding; double-stranded DNA binding; damaged DNA binding; chromatin binding; transcription factor activity; transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; G1 DNA damage checkpoint; cerebrospinal fluid secretion; positive regulation of cell size; activation of MAPK activity; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; negative regulation of cardiac muscle cell proliferation; response to gamma radiation; positive regulation of oligodendrocyte differentiation; negative regulation of neuron apoptosis; cell cycle arrest; kidney development; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; response to X-ray; negative regulation of JNK activity; release of cytochrome c from mitochondria; mismatch repair; hippocampus development; digestive tract morphogenesis; response to organic nitrogen; negative regulation of neuron differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of gene expression; neuron development; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus