Mouse anti-Human p53 Monoclonal Antibody | anti-p53 antibody

Anti-p53 Antibody

Cat. Num. AAA4156953

• Gene Names: TP53; P53; BCC7; LFS1; BMFS5; TRP53
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Protein A Purified
• Synonyms: p53; Monoclonal Antibody; Anti-p53 Antibody; anti-p53 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This protein A purified mousemonoclonal antibody reacts specifically with p53 in humantissues and cell lines. The antibody recognizes a 53 kDa bandcorresponding to p53. Cross reactivity with p53 from othersources has not been determined.
• Purity/Purification: Protein A Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-p53 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: This antibody is suitable for flow cytometry, immunoblotting andimmunoprecipitation. p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutationsarise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primarymelanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dentalfibroblasts following mild ultraviolet irradiation. This antibody reacts with a N-terminal epitope of the 53 kD gene productand this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-p53 antibody

The p53 gene like the Rb gene, is a tumor suppressor gene, i.e., its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are predisposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The p53 gene has been mapped to chromosome 17. In the cell, p53 protein binds DNA, which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division. Mutant p53 can no longer bind DNA in an effective way, and as a consequence the p21 protein is not made available to act as the 'stop signal' for cell division. Thus cells divide uncontrollably, and form tumors. p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Help with unraveling the molecular mechanisms of cancerous growth has come from the use of mice as models for human cancer, in which powerful 'gene knockout' techniques can be used. The amount of information that exists on all aspects of p53 normal function and mutant expression in human cancers is now vast, reflecting its key role in the pathogenesis of human cancers. It is clear that p53 is just one component of a network of events that culminate in tumor formation.

NCBI and Uniprot Product Information

• NCBI GI #: 23491729
• NCBI GeneID: 7157
• NCBI Official Full Name: P53
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; BMFS5; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• Protein Family: p53 and DNA damage-regulated protein

NCBI Description

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]