Mouse anti-Human P4HB Monoclonal Antibody | anti-P4HB antibody

Anti-P4HB Antibody (HRP), Mouse Monoclonal

Cat. Num. AAA8104118

• Gene Names: P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: P4HB; Monoclonal Antibody; Anti-P4HB Antibody (HRP); Mouse Monoclonal; P4HB/ERBA2L Antibody (HRP); Mouse MAb; prolyl 4-hydroxylase; beta polypeptide; Anti-DSI Antibody; Anti-ERBA2L Antibody; Anti-GIT Antibody; Anti-P4Hbeta Antibody; Anti-PDI Antibody; Anti-PDIA1 Antibody; Anti-PHDB Antibody; Anti-PO4DB Antibody; Anti-PO4HB Antibody; Anti-PROHB Antibody; anti-P4HB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 2F6G12H2
• Specificity: Human P4HB/ERBA2L
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-P4HB antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-1ug/ml
• Immunogen: Recombinant Human P4HB protein
• Conjugation: HRP
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human P4HB/ERBA2L (rh P4HB). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-P4HB antibody

Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

References

Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17. Pihlajaniemi T, et al., 1991, J Hepatol., 13, Suppl 3: S2 Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.

NCBI and Uniprot Product Information

• NCBI GI #: 20070125
• NCBI GeneID: 5034
• NCBI Accession #: NP_000909.2
• NCBI GenBank Nucleotide #: NM_000918.3
• UniProt Accession #: P07237; P30037; P32079; Q15205; Q6LDE5; B2RDQ2
• Molecular Weight: 508
• NCBI Official Full Name: protein disulfide-isomerase
• NCBI Official Synonym Full Names: prolyl 4-hydroxylase, beta polypeptide
• NCBI Official Symbol: P4HB
• NCBI Official Synonym Symbols: DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta
• NCBI Protein Information: protein disulfide-isomerase; p55; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protei
• UniProt Protein Name: Protein disulfide-isomerase
• UniProt Gene Name: P4HB
• UniProt Synonym Gene Names: ERBA2L; PDI; PDIA1; PO4DB; PDI
• UniProt Entry Name: PDIA1_HUMAN