Rat P2X7 Monoclonal Antibody | anti-P2X7 antibody

RAT ANTI MOUSE P2X7

Cat. Num. AAA219666

• Gene Names: P2rx7; P2X7R; P2X(7); AI467586
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: P2X7; Monoclonal Antibody; RAT ANTI MOUSE P2X7; anti-P2X7 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: Hano43
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 595

• Applicable Applications for anti-P2X7 antibody: Flow cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: A P2X7-expression construct and a final boost with P2X7-transfected HEK cells.; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Rat peritoneal macrophages stained with Rat anti Mouse P2X7 (MBS219666))

Testing Data #2

(Rat peritoneal macrophages stained with Rat anti Mouse P2X7:Alexa Fluor 488)

Related Product Information for anti-P2X7 antibody

Rat anti Mouse P2X7 antibody, clone Hano43 recognizes the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.

NCBI and Uniprot Product Information

• NCBI GI #: 84781766
• NCBI GeneID: 18439
• NCBI Accession #: NP_035157.2
• NCBI GenBank Nucleotide #: NM_011027.3
• UniProt Accession #: Q9Z1M0
• Molecular Weight: 68,388 Da
• NCBI Official Full Name: P2X purinoceptor 7 isoform a
• NCBI Official Synonym Full Names: purinergic receptor P2X, ligand-gated ion channel, 7
• NCBI Official Symbol: P2rx7
• NCBI Official Synonym Symbols: P2X7R; P2X(7); AI467586
• NCBI Protein Information: P2X purinoceptor 7; ATP receptor; P2X7 purinoceptor; P2X7 receptor; P2Z receptor
• UniProt Protein Name: P2X purinoceptor 7
• UniProt Gene Name: P2rx7
• UniProt Synonym Gene Names: P2x7; P2X7
• UniProt Entry Name: P2RX7_MOUSE

Uniprot Description

P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Receptor, misc.; Membrane protein, integral

Cellular Component: cell soma; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; integral to nuclear inner membrane; intercellular junction; intracellular; cytosol; neuromuscular junction; external side of plasma membrane

Molecular Function: protein binding; channel activity; lipopolysaccharide binding; ion channel activity; receptor activity; purinergic nucleotide receptor activity; ATP binding; ATP-gated cation channel activity

Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; positive regulation of T cell mediated cytotoxicity; activation of MAPK activity; cytolysis; positive regulation of glutamate secretion; programmed cell death; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; sensory perception of pain; response to organic cyclic substance; protein amino acid phosphorylation; positive regulation of interleukin-1 alpha secretion; multicellular organismal protein catabolic process; ceramide biosynthetic process; T cell homeostasis; response to electrical stimulus; positive regulation of gamma-aminobutyric acid secretion; response to drug; mitochondrion organization and biogenesis; collagen metabolic process; positive regulation of interleukin-6 production; protein oligomerization; phospholipid translocation; phagolysosome formation; membrane depolarization; defense response to Gram-positive bacterium; response to bacterium; NAD transport; positive regulation of protein secretion; response to zinc ion; response to mechanical stimulus; response to calcium ion; cell volume homeostasis; phospholipid transfer to membrane; cell morphogenesis; T cell proliferation; response to organic substance; positive regulation of mitochondrial depolarization; positive regulation of MAPKKK cascade; plasma membrane organization and biogenesis; transport; calcium ion transport; pore complex biogenesis; inflammatory response; negative regulation of bone resorption; cation transport; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; bleb formation; positive regulation of bone mineralization; response to ATP; positive regulation of ossification; positive regulation of interleukin-1 beta production; synaptic vesicle exocytosis; skeletal morphogenesis; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; positive regulation of prostaglandin secretion; cellular response to extracellular stimulus; gene expression; protein processing; ion transport; positive regulation of protein amino acid phosphorylation; membrane budding; positive regulation of cytokine secretion