Mouse p27kip1 Monoclonal Antibody

p27kip1, phosphorylated (Thr187)

Cat. Num. AAA632206

• Reactivity: Hamster, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A chromatography.
• Synonyms: p27kip1; Monoclonal Antibody; phosphorylated (Thr187); Anti -p27kip1; anti-p27kip1 antibody

• Host: Mouse
• Reactivity: Hamster, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6A153
• Specificity: Specific for p27 when phosphorylated at Thr187. On Western blots, it identifies the target band at ~27kD. It does not react with unphosphorylated p27. Specificity has been confirmed with Thr187-mutant p27 recombinant protein. Reactivity has been confirmed with p27-transfected 293 cells. Based on sequence homology, this antibody may also react with mouse, rat and hamster.
• Purity/Purification: Affinity Purified; Purified by Protein A chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide

• Applicable Applications for anti-p27kip1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.1-1ug/ml; Western Blot: 1-3ug/ml
• Immunogen: Synthetic peptide encompassing the phosphorylated Thr187 residue of human p27
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-p27kip1 antibody

The cyclin-dependent kinase inhibitor p27KIP1 is a potent inhibitor of both Cyclin E-Cdk2 and Cyclin A-Cdk2, two cdk complexes involved in the regulation of G1/S transition. p27 is phosphorylated on threonine 187 by cyclin E/Cdk2, and overexpression of this complex in mammalian cells induces degradation of transfected p27. The Cdk-mediated phosphorylation of p27 on threonine 187 is required for p27 ubiquitination and consequent degradation. The phosphorylation on threonine 187 and the ubiquitination of cellular p27, as well as p27 ubiquitinating activity, were found to be regulated collectively during the cell cycle, being present in proliferating cells but not in G0- and G1- arrested cells.

Product Categories/Family for anti-p27kip1 antibody

Antibodies; Abs to Protein Kinase Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 4587316
• NCBI Official Full Name: p27kip1

NCBI Description

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Uniprot Description

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Protein type: Cell cycle regulation; Oncoprotein; Inhibitor; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12p13.1-p12

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; endosome

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; chaperone binding; protein complex binding; Hsp70 protein binding; caspase activator activity; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: response to peptide hormone stimulus; negative regulation of kinase activity; nerve growth factor receptor signaling pathway; positive regulation of microtubule polymerization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to estradiol stimulus; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; response to glucose stimulus; negative regulation of mitotic cell cycle; cell cycle arrest; inner ear development; potassium ion transport; response to drug; caspase activation; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; response to amino acid stimulus; response to cadmium ion; response to hypoxia; positive regulation of protein catabolic process; autophagic cell death; negative regulation of phosphorylation; innate immune response; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

Disease: Multiple Endocrine Neoplasia, Type Iv