Mouse anti-Human p21Cip1/WAF1 Monoclonal Antibody | anti-PPM1G antibody

p21Cip1/WAF1

Cat. Num. AAA601809

• Gene Names: PPM1G; PP2CG; PPP2CG; PP2CGAMMA
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: p21Cip1/WAF1; Monoclonal Antibody; Anti -p21Cip1/WAF1; anti-PPM1G antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G223
• Specificity: Recognizes an epitope formed by aa58-77 of human p21Cip1/WAF1. Species Crossreactivity: Does not react with the corresponding mouse or rat proteins.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied s a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-PPM1G antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Immunogen: Full length recombinant human p21Cip1/WAF1.
• Preparation and Storage: -20 degree C

Related Product Information for anti-PPM1G antibody

p21Cip1/WAF1 (p21) is an inhibitor of cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. The p21 promoter contains a p53-binding site and transcriptional activation can occur through wild-type but not mutant p53. Induction of p21 has been shown to be essential for p53-mediated arrest of the cell cycle in G1 in response to DNA damage, and transfection of p21 into both tumor cell lines and normal fibroblasts results in growth inhibition. Although expression of p21 has been demonstrated to be largely dependent upon p53, p53-independent expression of p21 has also been observed. Simultaneously with its discovery as a p53 regulated gene, p21 was also shown to be a potent inhibitor of G1 cyclin dependent kinases. P21 can associate with every cyclin kinase complex examined; however, the protein appears to preferentially inhibit CDK/cyclin complexes that play a role in the G1/S transition. Thus, p21 has been postulated to act predominantly as a cell cycle buffer which must be titrated in order to exit the G1 phase of the cell cycle. In addition to its role as a CDK inhibitor, p21 has been shown to bind and inactivate components of the DNA replication machinery.

Product Categories/Family for anti-PPM1G antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 29826282
• NCBI GeneID: 5496
• NCBI Accession #: NP_817092.1
• NCBI GenBank Nucleotide #: NM_177983.2
• UniProt Accession #: O15355
• Molecular Weight: 59,272 Da
• NCBI Official Full Name: protein phosphatase 1G
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1G
• NCBI Official Symbol: PPM1G
• NCBI Official Synonym Symbols: PP2CG; PPP2CG; PP2CGAMMA
• NCBI Protein Information: protein phosphatase 1G; PP2C-gamma; PP2C, gamma; protein phosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• UniProt Protein Name: Protein phosphatase 1G
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1G
• UniProt Synonym Gene Names: PPM1C; PP2C-gamma
• UniProt Entry Name: PPM1G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Uniprot Description

PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Spliceosome; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: cell cycle arrest; protein amino acid dephosphorylation