Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat PCSK6 Polyclonal Antibody | anti-PCSK6 antibody
Goat anti-PCSK6 Antibody
Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows striated staining in alternating fibres. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments gave an approx 40-45 kDa band in Human Brain (Cerebellum) lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106 kDa according to NP_002561.1. The 40-45 kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]
Uniprot Description
PCSK6: Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; EC 3.4.21.-; Secreted, signal peptide; Protease
Chromosomal Location of Human Ortholog: 15q26.3
Cellular Component: cell surface; endoplasmic reticulum; extracellular matrix; extracellular space; Golgi lumen; membrane
Molecular Function: endopeptidase activity; heparin binding; nerve growth factor binding; serine-type endopeptidase activity
Biological Process: determination of left/right symmetry; glycoprotein metabolic process; nerve growth factor processing; nerve growth factor production; peptide hormone processing; protein processing; regulation of BMP signaling pathway; secretion by cell; zygotic determination of anterior/posterior axis, embryo
Research Articles on PCSK6
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Product Notes
The PCSK6 pcsk6 (Catalog #AAA421630) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PCSK6 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCSK6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows striated staining in alternating fibres. Recommended concentration, 5-10ug/ml. Western Blot: Preliminary experiments gave an approx 40-45 kDa band in Human Brain (Cerebellum) lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106 kDa according to NP_002561.1. The 40-45 kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the PCSK6 pcsk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: YHTFSAHQSR SR. It is sometimes possible for the material contained within the vial of "PCSK6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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