Mouse anti-Human Osteopontin Monoclonal Antibody | anti-SPP1 antibody

Mouse anti Human Osteopontin:Biotin

Cat. Num. AAA226220

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: Osteopontin; Monoclonal Antibody; Mouse anti Human Osteopontin:Biotin; Osteopontin antibody | F03-4E8; OSTEOPONTIN; anti-SPP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: F03-4E8
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Purified IgG conjugated to Biotin - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-SPP1 antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: HEK293 derived recombinant human full length osteopontin (Ile17-Asn314)
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Conjugation: Biotin
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human OPN using Mouse anti Human osteopontin as a capture reagent and biotinylated Mouse anti Human osteopontin (MBS226220) as a detection reagent with purified recombinant OPN as the antigen. Detection was performed )

Related Product Information for anti-SPP1 antibody

Mouse anti Human osteopontin antibody, clone F03-4E8, recognizes osteopontin (OPN) also known as bone sialoprotein 1, nephropontin secreted phosphoprotein 1 and uropontin. It is an acidic glycoprotein and is a member of the intrinsically disordered class of proteins (Kurzbach et al. 2013). OPN plays a role in a multitude of physiological and pathophysiological processes. In inflammation it induces cell adhesion and migration, regulates differentiation of proinflammatory lymphocytes and inhibits apoptosis of inflammatory cells (Clemente et al. 2016). OPN is expressed by various cell types including bone, neural, epithelial and endothelial cells. It is also expressed by the immune cells T cells, B cells, natural killer cells and macrophages. In its secreted form OPN can be found in biological fluids such as blood, urine, bile and milk (Castello et al. 2017). OPN has been found to play a role in cancer progression by enhancing proliferation, survival, motility and invasion of tumor cells in a range of cancers. OPN was upregulated at tumor sites and in blood of the patients, with the level correlating with tumor stage and aggressiveness (Castello et al. 2017). High levels of OPN have also been detected in several autoimmune diseases including multiple sclerosis and rheumatoid arthritis (Clemente et al. 2016).

The biotinylated Mouse anti Human osteopontin antibody, clone F03-4E8 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human osteopontin antibody, clone F11-4C11 as the capture antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4759166
• NCBI GeneID: 6696
• NCBI Accession #: NP_000573.1
• NCBI GenBank Nucleotide #: NM_000582.2
• UniProt Accession #: P10451; Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1
• Molecular Weight: 35,423 Da
• NCBI Official Full Name: osteopontin isoform OPN-b
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1
• NCBI Protein Information: osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I,
• UniProt Protein Name: Osteopontin
• Protein Family: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1
• UniProt Entry Name: OSTP_HUMAN

NCBI Description

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region

Molecular Function: protein binding; extracellular matrix binding; cytokine activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; decidualization; osteoblast differentiation; extracellular matrix disassembly; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; inflammatory response; cell adhesion; embryo implantation