Mouse anti-Human ODC1 Monoclonal Antibody | anti-ODC1 antibody

ODC1 (Ornithine Decarboxylase, ODC) (HRP)

Cat. Num. AAA6153910

• Gene Names: ODC1; ODC
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ODC1; Monoclonal Antibody; ODC1 (Ornithine Decarboxylase; ODC) (HRP); anti-ODC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2G5
• Specificity: Recognizes human ODC1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-ODC1 antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-461 from human ODC1 (AAH25296) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGAGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ODC1 expression in transfected 293T cell line by ODC1 monoclonal antibody. Lane 1: ODC1 transfected lysate (51.148kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ODC1 on HeLa cell. [antibody concentration 10ug/ml])

Immunoprecipitation (IP)

(Immunoprecipitation of ODC1 transfected lysate using ODC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ODC1 rabbit polyclonal antibody.)

Related Product Information for anti-ODC1 antibody

ODC1, is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. ODC1 plays an important role in diverse biological processes, including cell growth, differentiation, transformation and apoptosis. Overexpression of the ODC1 gene plays important roles in cell proliferation and the development of cancer.

Product Categories/Family for anti-ODC1 antibody

Antibodies; Enzymes; Decarboxylase

NCBI and Uniprot Product Information

• NCBI GI #: 19263695
• NCBI GeneID: 4953
• UniProt Accession #: P11926; Q53TU3; Q6LDS9
• Molecular Weight: 51,148 Da
• NCBI Official Full Name: Homo sapiens ornithine decarboxylase 1, mRNA
• NCBI Official Synonym Full Names: ornithine decarboxylase 1
• NCBI Official Symbol: ODC1
• NCBI Official Synonym Symbols: ODC
• NCBI Protein Information: ornithine decarboxylase
• UniProt Protein Name: Ornithine decarboxylase
• Protein Family: Mitochondrial 2-oxodicarboxylate carrier
• UniProt Gene Name: ODC1
• UniProt Synonym Gene Names: ODC
• UniProt Entry Name: DCOR_HUMAN

NCBI Description

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2013]

Uniprot Description

ODC: the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq, Jul 2008]

Protein type: EC 4.1.1.17; Amino Acid Metabolism - arginine and proline; Other Amino Acids Metabolism - glutathione; Cell cycle regulation; Lyase

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; ornithine decarboxylase activity

Biological Process: response to virus; positive regulation of cell proliferation; regulation of protein catabolic process; kidney development; regulation of amino acid metabolic process; putrescine biosynthetic process from ornithine; polyamine metabolic process

Disease: Colorectal Cancer