Mouse anti-Human Nucleoprotein Interactor-1, hSRP-1 Monoclonal Antibody
Nucleoprotein Interactor-1, hSRP-1 (NPI-1)
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A chromatography
Synonyms
Nucleoprotein Interactor-1; hSRP-1; Monoclonal Antibody; hSRP-1 (NPI-1); Anti -Nucleoprotein Interactor-1; anti-Nucleoprotein Interactor-1; hSRP-1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6A145
Specificity
Specific for the NPI-1 (nucleoprotein interactor-1, karyopherin alpha 1, hSRP1) protein. On Western blots, it identifies a band at ~60kD. Reactivity has been confirmed with human HeLa and MDBK cell lysates.
Purity/Purification
Affinity Purified
Purified by Protein A chromatography
Purified by Protein A chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide
Applicable Applications for anti-Nucleoprotein Interactor-1, hSRP-1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunoprecipitation, Immunofluorescence, ELISA and Western Blot. Other applications have not been tested
Dilution: Immunoprecipitation (native): 5-10ug
Immunofluorescence: 5-10ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Dilution: Immunoprecipitation (native): 5-10ug
Immunofluorescence: 5-10ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunogen
GST fusion protein
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Nucleoprotein Interactor-1, hSRP-1 antibody
Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. NPI-1 (nucleoprotein interactor-1, hSRP-1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal. Both subunits of karyopherin are required for protein docking at the NPC; the alpha subunit binds the substrate and the beta subunit acts as an adaptor. Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin beta and promotes the release of both subunits from the docking site. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC.
Product Categories/Family for anti-Nucleoprotein Interactor-1, hSRP-1 antibody
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Product Notes
The Nucleoprotein Interactor-1, hSRP-1 (Catalog #AAA601602) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nucleoprotein Interactor-1, hSRP-1 (NPI-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoprotein Interactor-1, hSRP-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in Immunoprecipitation, Immunofluorescence, ELISA and Western Blot. Other applications have not been tested Dilution: Immunoprecipitation (native): 5-10ug Immunofluorescence: 5-10ug/ml ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml. Researchers should empirically determine the suitability of the Nucleoprotein Interactor-1, hSRP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleoprotein Interactor-1, hSRP-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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