Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51. Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.)
Mouse anti-Mouse, Rat NrCAM Monoclonal Antibody | anti-Nrcam antibody
NrCAM Antibody: Alkaline Phosphatase
Gene Names
Nrcam; Bravo; mKIAA0343; C030017F07Rik; C130076O07Rik
Reactivity
Mouse, Rat
Applications
Western Blot, Immunocytochemistry
Synonyms
NrCAM; Monoclonal Antibody; NrCAM Antibody: Alkaline Phosphatase; Neuronal cell adhesion molecule; Bravo; hBravo; KIAA0343; MGC138845; MGC138846; Neuronal surface protein Bravo; Ng CAM related; Ng-CAM-related; NgCAM related cell adhesion molecule; NgCAM-related cell adhesion molecule; Nr CAM; Nr-CAM; anti-Nrcam antibody
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
S364-51
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
1186
Applicable Applications for anti-Nrcam antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
1:1000 WB
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51. Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51. Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.)
Related Product Information for anti-Nrcam antibody
Background Info: Detects a 160 kDa protein.
Scientific Background: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.
Scientific Background: Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.
Product Categories/Family for anti-Nrcam antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,964 Da
NCBI Official Full Name
neuronal cell adhesion molecule isoform 2
NCBI Official Synonym Full Names
neuronal cell adhesion molecule
NCBI Official Symbol
Nrcam
NCBI Official Synonym Symbols
Bravo; mKIAA0343; C030017F07Rik; C130076O07Rik
NCBI Protein Information
neuronal cell adhesion molecule; mBravo; nr-CAM; ng-CAM-related; neuronal surface protein Bravo; ngCAM-related cell adhesion molecule; neuron-glia-CAM-related cell adhesion molecule
UniProt Protein Name
Neuronal cell adhesion molecule
Protein Family
UniProt Gene Name
Nrcam
UniProt Synonym Gene Names
Kiaa0343; Nr-CAM; mBravo; Ng-CAM-related
UniProt Entry Name
NRCAM_MOUSE
Uniprot Description
NRCAM: Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier. Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis
Cellular Component: membrane; axon; plasma membrane; integral to membrane; synapse
Molecular Function: protein binding; ankyrin binding
Biological Process: heterotypic cell-cell adhesion; clustering of voltage-gated sodium channels; axon guidance; cell-cell adhesion; protein localization; central nervous system development; action potential propagation; cell adhesion; retinal ganglion cell axon guidance
Research Articles on Nrcam
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Product Notes
The Nrcam nrcam (Catalog #AAA803228) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NrCAM Antibody: Alkaline Phosphatase reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NrCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). 1:1000 WB. Researchers should empirically determine the suitability of the Nrcam nrcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NrCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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