Mouse anti-Human NR1H3 Monoclonal Antibody | anti-NR1H3 antibody

NR1H3 (Oxysterols Receptor LXR-alpha, Liver X Receptor alpha, Nuclear Receptor Subfamily 1 Group H Member 3, LXRA) APC

Cat. Num. AAA6137946

• Gene Names: NR1H3; LXRA; LXR-a; RLD-1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR1H3; Monoclonal Antibody; NR1H3 (Oxysterols Receptor LXR-alpha; Liver X Receptor alpha; Nuclear Receptor Subfamily 1 Group H Member 3; LXRA) APC; anti-NR1H3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3E9
• Specificity: Recognizes human NR1H3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-NR1H3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant protein corresponding to aa1-387 from NR1H3 (AAH08819) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSLWLGAPVPDIPPDSAVELWKPGAQDASSQAQGGSSCILREEARMPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (68.31kD).)

Western Blot (WB)

(Western Blot analysis of NR1H3 expression in transfected 293T cell line by NR1H3 monoclonal antibody. Lane 1: NR1H3 transfected lysate (45.7kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged NR1H3 is 3ng/ml as a capture antibody.)

Related Product Information for anti-NR1H3 antibody

Liver X receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous.

Product Categories/Family for anti-NR1H3 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 33873958
• NCBI GeneID: 10062
• Molecular Weight: 45,692 Da
• NCBI Official Full Name: Homo sapiens nuclear receptor subfamily 1, group H, member 3, mRNA
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1 group H member 3
• NCBI Official Symbol: NR1H3
• NCBI Official Synonym Symbols: LXRA; LXR-a; RLD-1
• NCBI Protein Information: oxysterols receptor LXR-alpha
• Protein Family: Oxysterols receptor

NCBI Description

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]