Mouse anti-Human NPM1 Monoclonal Antibody | anti-NPM1 antibody

NPM1 Mouse mAb

Cat. Num. AAA435104

• Gene Names: NPM1; B23; NPM
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: NPM1; Monoclonal Antibody; NPM1 Mouse mAb; anti-NPM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 259

• Applicable Applications for anti-NPM1 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human NPM
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

(All lanes : NPM Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 33 KDa Observed band size : 33 KDa Blocking/Dilution buffer : 1x TBST.)

Immunoprecipitation (IP)

(NPM was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25053 of 10 ug. Western blot was performed from the immunoprecipitate using M25053 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NPM with M25053 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Related Product Information for anti-NPM1 antibody

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF, p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants

Product Categories/Family for anti-NPM1 antibody

Cell Marker; Subcellular Marker; Nucleoplasma

NCBI and Uniprot Product Information

• NCBI GI #: 83641870
• NCBI GeneID: 4869
• NCBI Accession #: NP_001032827.1
• NCBI GenBank Nucleotide #: NM_001037738.2
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: Predicted MW: 33kDa
• NCBI Official Full Name: nucleophosmin isoform 3
• NCBI Official Synonym Full Names: nucleophosmin 1
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM

NCBI Description

The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]

Uniprot Description

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).